Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CPEB polyclonal antibody (Product # PA1-1100) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C H(545) S M E G L R H H S P L M R N Q K N(562) of human CPEB.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1100 detects recombinant mouse and rat CPEB (cytoplasmic polyadenylation element binding protein).
PA1-1100 has been successfully used in Western blot, ICC/IF and IHC-P procedures. By Western blot, this antibody detects ~62 and kDa protein representing recombinant mouse CPEB.
The PA1-1100 immunogen is a synthetic peptide corresponding to residues C H(545) S M E G L R H H S P L M R N Q K N(562) of human CPEB. This sequence is completely conserved in mouse. The PA1-1100 immunizing peptide (Cat. # PEP-171) is available for use in neutralization and control experiments.
CPEB1 is an RNA binding protein that contains an RNA-recognition motif and a zinc finger-containing region found in a wide range of vertebrates and invertebrates. CPEB1 forms the nucleus of a complex of factors that regulate poly(A) elongation and promotes polyadenylation-induced translation. CPEB1 mediates many diverse biological processes such as germ cell development, cell division and senescence, and synaptic plasticity. Recently, it was discovered that CPEB1 is involved in beta-catenin mRNA translation and cell migration in astrocytes as well as regulating hypoxia-inducible factor (HIF)-1 expression, demonstrating the wide range of processes in which CPEB1 plays a role. At least four isoforms of CPEB1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells.
PA1-1100 was used in immunoprecipitation to study the mechanism by which knockdown of CPEB1 promotes mammary epithelial cell differentiation and blocks epithelial-to-mesenchymal transition
|Grudzien-Nogalska E,Reed BC,Rhoads RE||Journal of cell science (127:2326)||2014|
Time of day regulates subcellular trafficking, tripartite synaptic localization, and polyadenylation of the astrocytic Fabp7 mRNA.
PA1-1100 was used in immunoprecipitation to investigate the circadian control of astrocytic Fabp7 mRNA trafficking and subsequent translation
|Gerstner JR,Vanderheyden WM,LaVaute T,Westmark CJ,Rouhana L,Pack AI,Wickens M,Landry CF||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:1383)||2012|
Proteasomal activity is required to initiate and to sustain translational activation of messenger RNA encoding the stem-loop-binding protein during meiotic maturation in mice.
PA1-1100 was used in western blot to test whether CPEB degradation is required for translational activation of Slbp mRNA
|Yang Q,Allard P,Huang M,Zhang W,Clarke HJ||Biology of reproduction (82:123)||2010|