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|Tested species reactivity||Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Purified yeast CPY (carboxypeptidase)|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 µg/ml|
|Immunofluorescence (IF)||20 µg/ml|
|Western Blot (WB)||0.25 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.
Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe.
A-6428 was used in western blot to study the regulation of cell wall synthesis by the clathrin light chain.
|de León N,Sharifmoghadam MR,Hoya M,Curto MÁ,Doncel C,Valdivieso MH||PloS one (8:null)||2013|
Altered distribution of the yeast plasma membrane H+-ATPase as a feature of vacuolar H+-ATPase null mutants.
A-6428 was used in western blot to assess the effect of vacuolar H(+)-ATPase null mutations on the targeting of the plasma membrane H(+)-ATPase through the secretory pathway.
|Perzov N,Nelson H,Nelson N||The Journal of biological chemistry (275:40088)||2000|
Carboxypeptidase Y, pcy1, cpy1