Invitrogen

CRABP2 Recombinant Rabbit Monoclonal Antibody (4)

View all (33) CRABP2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CRABP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

CRABP2 Antibody in Western Blot (WB) — Western Blot using CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 14 kDa.

CRABP2 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual, stained with CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

CRABP2 Antibody in Immunoprecipitation (IP) — CRABP2 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg Raji Whole Cell Lysate 0.5 µL with CRABP2 Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29188) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: CRABP2 Recombinant Rabbit Monoclonal Antibody (4), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDa.

Product Details

MA5-29188

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

1:100-1:500

Immunoprecipitation (IP)

0.1-0.5 µL/mg of lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

Immunogen

Recombinant Human CRABP2 protein (Pro2-Glu138)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

RRID

AB_2785100

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human CRABP2.

Target Information

Cellular retinoic acid binding protein (CRABP) is a low molecular weight protein with unknown function and existing in two isoforms, CRABP1 and CRABP2. The predicted amino acid sequences of human CRABP1 and CRABP2 exhibits 99.3 and 93.5% identity to mouse CRABP1 and CRABP2, respectively. The CRABP2 isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. The CRABP2 gene localized on human chromosome 1q21.3 is transcriptionally regulated by a newly synthesized regulatory protein. The protein is expressed in human adult liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cellular retinoic acid-binding protein 2; Cellular retinoic acid-binding protein II; CRABP-II; RP11-66D17.4

Gene Aliases: CRABP-II; CRABP2; RBP6

UniProt ID: (Human) P29373

Entrez Gene ID: (Human) 1382

Function(s)

regulation of transcription, DNA-templated transport signal transduction epidermis development embryonic forelimb morphogenesis retinoic acid metabolic process regulation of retinoic acid receptor signaling pathway positive regulation of collateral sprouting

Process(es)

retinoic acid binding transporter activity retinoid binding protein binding retinal binding retinol binding