Invitrogen

CRABP2 Monoclonal Antibody (5CRA-3B3)

Product Image Gallery

CRABP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CRABP2 (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a CRABP2 monoclonal antibody (Product # MA3-017) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Secondary Antibody, DyLight 488 conjugate (Product # 35502) at a dilution of 1:500 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

CRABP2 Antibody in Western Blot (WB) — Western blot analysis of CRABP2 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CRABP2 was detected at ~14 kD using a CRABP2 monoclonal antibody (Product # MA3-017) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Product Details

MA3-017

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	5CRA-3B3
Immunogen	recombinant protein corresponding to full length human CRABPII View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2609038

Product Specific Information

MA3-017 has been used successfully in Western blotting and Immunofluorescence. This antibody was referenced in publications to perform in IHC.

Target Information

Cellular retinoic acid binding protein (CRABP) is a low molecular weight protein with unknown function and existing in two isoforms, CRABP1 and CRABP2. The predicted amino acid sequences of human CRABP1 and CRABP2 exhibits 99.3 and 93.5% identity to mouse CRABP1 and CRABP2, respectively. The CRABP2 isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. The CRABP2 gene localized on human chromosome 1q21.3 is transcriptionally regulated by a newly synthesized regulatory protein. The protein is expressed in human adult liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.