Invitrogen

CRABP2 Monoclonal Antibody (5CRA-3B3)

View all (32) CRABP2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (2)

CRABP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

CRABP2 Antibody (MA3-017) in ICC/IF

Immunofluorescent analysis of CRABP2 (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a CRABP2 monoclonal antibody (Product # MA3-017) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Secondary Antibody, DyLight 488 conjugate (Product # 35502) at a dilution of 1:500 fo... View More

Product Image Gallery

CRABP2 Antibody in Western Blot (WB) — Western blot analysis of CRABP2 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CRABP2 was detected at ~14 kD using a CRABP2 monoclonal antibody (Product # MA3-017) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Product Details

MA3-017

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunocytochemistry (ICC/IF)

1:50 - 1:200

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

5CRA-3B3

Immunogen

recombinant protein corresponding to full length human CRABPII

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2609038

Product Specific Information

MA3-017 has been used successfully in Western blotting and Immunofluorescence. This antibody was referenced in publications to perform in IHC.

Target Information

Cellular retinoic acid binding protein (CRABP) is a low molecular weight protein with unknown function and existing in two isoforms, CRABP1 and CRABP2. The predicted amino acid sequences of human CRABP1 and CRABP2 exhibits 99.3 and 93.5% identity to mouse CRABP1 and CRABP2, respectively. The CRABP2 isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. The CRABP2 gene localized on human chromosome 1q21.3 is transcriptionally regulated by a newly synthesized regulatory protein. The protein is expressed in human adult liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Cellular retinoic acid-binding protein 2; Cellular retinoic acid-binding protein II; CRABP-II; RP11-66D17.4

Gene Aliases: CRABP-II; CRABP2; RBP6

UniProt ID: (Human) P29373

Entrez Gene ID: (Human) 1382

Function(s)

regulation of transcription, DNA-templated transport signal transduction epidermis development embryonic forelimb morphogenesis retinoic acid metabolic process regulation of retinoic acid receptor signaling pathway positive regulation of collateral sprouting

Process(es)

retinoic acid binding transporter activity retinoid binding protein binding retinal binding retinol binding