Immunofluorescent analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) (Product# MA3-813) shows staining in NIH-3T3 Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI (Product# MA3-813) at a dilution of 1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Chicken, Cat, Human, Mouse, Non-human primate, Rabbit, Reptile, Rat|
|Published species reactivity||Avian, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Oxidized CRABPI from bovine retina.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1-2ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||1:250|
|Immunohistochemistry (Paraffin) (IHC (P))||1/1000|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-813 detects cellular retinoic acid binding protein I (CRABPI) from human, mouse, monkey, feline, bovine, rabbit, rat, turtle and chicken tissues. This antibody does not react with chameleon, goldfish and frog. This antibody does not cross-react with cellular retinol binding protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein.
MA3-813 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 16 kDa protein representing CRABPI from rat retinal supernatant. Immunohistochemical staining of CRABPI in rat retina with MA3-813 results in its localization to amacrine somata and laminae in the inner plexiform layer.
The MA3-813 antigen is oxidized CRABPI from bovine retina.
Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The cellular retinoic acid binding proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins.
Two forms of CRABP have been characterized, CRABPI and CRABPII which share ~75% sequence conservation. The main role of CRABPI may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABPII is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proteome analysis of the effects of all-trans retinoic acid on human germ cell tumor cell lines.
MA3-813 was used in western blot to study the effects all-trans retinoic on human germ cell tumor cells using proteomics
|Honecker F,Rohlfing T,Harder S,Braig M,Gillis AJ,Glaesener S,Barett C,Bokemeyer C,Buck F,Brümmendorf TH,Looijenga LH,Balabanov S||Journal of proteomics (96:300)||2014|
Retinoic acid orchestrates fibroblast growth factor signalling to drive embryonic stem cell differentiation.
MA3-813 was used in western blot to investigate the effect of retinoic acid on embryonic stem cell differentiation
|Stavridis MP,Collins BJ,Storey KG||Development (Cambridge, England) (137:881)||2010|
LIF removal increases CRABPI and CRABPII transcripts in embryonic stem cells cultured in retinol or 4-oxoretinol.
MA3-813 was used in western blot to study the effect of leukemia inhibitory factor on CRABPI and CRABPII transcription in embryonic stem cells
|Lane MA,Xu J,Wilen EW,Sylvester R,Derguini F,Gudas LJ||Molecular and cellular endocrinology (280:63)||2008|
|Not Applicable||Not Cited||
In cerebrospinal fluid ER chaperones ERp57 and calreticulin bind beta-amyloid.
MA3-813 was used in western blot to elucidate ER chaperones calreticulin and ERp57 bind beta-amyloid in cerebrospinal fluid
|Erickson RR,Dunning LM,Olson DA,Cohen SJ,Davis AT,Wood WG,Kratzke RA,Holtzman JL||Biochemical and biophysical research communications (332:50)||2005|
The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture.
MA3-813 was used in western blot to study the involvement of retinoic acid in the development of organ of Corti
|Kelley MW,Xu XM,Wagner MA,Warchol ME,Corwin JT||Development (Cambridge, England) (119:1041)||1993|
Isolation and properties of an in vitro human outer blood-retinal barrier model.
MA3-813 was used in immunocytochemistry to characterize an invitro human outer blood-retinal barrier model
|Hamilton RD,Leach L||Methods in molecular biology (Clifton, N.J.) (686:401)||2010|
SFRP1 modulates retina cell differentiation through a beta-catenin-independent mechanism.
MA3-813 was used in immunocytochemistry to investigate the mechanism for the effect of SFRP1 on retina cell differentiation
|Esteve P,Trousse F,Rodríguez J,Bovolenta P||Journal of cell science (116:2471)||2003|
Immunolocalization of cellular retinol-, retinaldehyde- and retinoic acid-binding proteins in rat retina during pre- and postnatal development.
MA3-813 was used in immunocytochemistry to investigate the cellular distribution of CRBP, CRALBP and CRABP in rat retina
|De Leeuw AM,Gaur VP,Saari JC,Milam AH||Journal of neurocytology (19:253)||1990|
Establishment of a human in vitro model of the outer blood-retinal barrier.
MA3-813 was used in immunohistochemistry to establish a human in vitro model of the outer blood-retinal barrier
|Hamilton RD,Foss AJ,Leach L||Journal of anatomy (211:707)||2007|
Differential gene expression in ovarian carcinoma: identification of potential biomarkers.
MA3-813 was used in immunohistochemistry to investigate the diagnostic, therapeutic, and prognostic biomarkers in ovarian carcinoma
|Hibbs K,Skubitz KM,Pambuccian SE,Casey RC,Burleson KM,Oegema TR,Thiele JJ,Grindle SM,Bliss RL,Skubitz AP||The American journal of pathology (165:397)||2004|
Retinoic acid promotes differentiation of photoreceptors in vitro.
MA3-813 was used in immunohistochemistry to define the involvement of retinoic acid in the development of the nervous system
|Kelley MW,Turner JK,Reh TA||Development (Cambridge, England) (120:2091)||1994|