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|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant CRALBP.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||1 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.02 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-813 detects CRALBP protein in bovine, human, mouse, non-human primate and rat samples.
MA1-813 has successfully been used in Western blot, immunohistochemistry, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects a 37 kDa protein representing human recombinant CRALBP.
The MA1-813 immunogen is purified human recombinant CRALBP.
Cellular retinaldehyde-binding protein (CRALBP) plays an important role in the regeneration of 11-cis-retinal for use in rod visual pigments such as opsin and rhodopsin. Once 11-cis-retinal is photoisomerized in the rod outer segment, it is converted to all-trans-retinal and further modified into all-trans-retinol. All-trans-retinol then diffuses into the retinal pigment epithelium (RPE) to be converted back to 11-cis-retinol and further oxidized into 11-cis-retinal (both by CRALBP). Genetic mutations involving CRALBP"e;s lack of function have been linked to visual disease such as bothnia dystrophy, retinitis punctata albescens, retina pigmentosa, and Newfoundland rod-cone dystrophy. The presence of CRALBP serves as a marker for RPE and Muller glial cells of the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle.
MA1-813 was used in immunocytochemistry and western blot to study induced pluripotent stem cell-derived retinal pigmented epithelial cells for visual cycle function and sutability for transplantation
|Muñiz A,Greene WA,Plamper ML,Choi JH,Johnson AJ,Tsin AT,Wang HC||Investigative ophthalmology & visual science (55:198)||2014|
GABA-mediated induction of early neuronal markers expression in postnatal rat progenitor cells in culture.
MA1-813 was used in immunocytochemistry to study the response to GABA-mediated signaling of retinal progenitors derived from Müller glia
|Ramírez M,Hernández-Montoya J,Sánchez-Serrano SL,Ordaz B,Ferraro S,Quintero H,Peña-Ortega F,Lamas M||Neuroscience (224:210)||2012|
Diabetes-induced peroxynitrite impairs the balance of pro-nerve growth factor and nerve growth factor, and causes neurovascular injury.
MA1-813 was used in immunocytochemistry to investigate the mechanism for neurovascular dysfunction induced by oxidative stress
|Ali TK,Al-Gayyar MM,Matragoon S,Pillai BA,Abdelsaid MA,Nussbaum JJ,El-Remessy AB||Diabetologia (54:657)||2011|
Genetic deletion of COX-2 diminishes VEGF production in mouse retinal Müller cells.
MA1-813 was used in immunocytochemistry to investigate the role of COX2 in vascular endothelial growth factor production in retinal Muller cells
|Yanni SE,McCollum GW,Penn JS||Experimental eye research (91:34)||2010|
Loss of Müller's cells and photoreceptors in macular telangiectasia type 2.
MA1-813 was used in immunohistochemistry to report on the clinicopathology of a patient with macular telangiectasia type 2
|Powner MB,Gillies MC,Zhu M,Vevis K,Hunyor AP,Fruttiger M||Ophthalmology (120:2344)||2013|
Ghrelin modulates physiologic and pathologic retinal angiogenesis through GHSR-1a.
MA1-813 was used in immunohistochemistry to investigate the role of ghrelin in retinal vasculariztaion
|Zaniolo K,Sapieha P,Shao Z,Stahl A,Zhu T,Tremblay S,Picard E,Madaan A,Blais M,Lachapelle P,Mancini J,Hardy P,Smith LE,Ong H,Chemtob S||Investigative ophthalmology & visual science (52:5376)||2011|
Perifoveal müller cell depletion in a case of macular telangiectasia type 2.
MA1-813 was used in immunohistochemistry to report a clinical case of macular telangiectasia type 2 with perifoveal m
|Powner MB,Gillies MC,Tretiach M,Scott A,Guymer RH,Hageman GS,Fruttiger M||Ophthalmology (117:2407)||2010|
Neuroprotection in the juvenile rat model of light-induced retinopathy: evidence suggesting a role for FGF-2 and CNTF.
MA1-813 was used in immunohistochemistry to investigate the role of FGF-2 and CNTF in protecting the juvenile retina from light-induced damage
|Joly S,Pernet V,Chemtob S,Di Polo A,Lachapelle P||Investigative ophthalmology & visual science (48:2311)||2007|
Temporal/spatial expression of retinoid binding proteins and RAR isoforms in the postnatal lung.
MA1-813 was used in immunohistochemistry to study components of the retinoid-signaling pathway during postnatal alveologenesis in the mouse
|Hind M,Corcoran J,Maden M||American journal of physiology. Lung cellular and molecular physiology (282:L468)||2002|
Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells.
MA1-813 was used in flow cytometry to develop in vitro methods for the highly sensitive detection of residual undifferentiated induced pluripotent stem cells following differentiation to retinal pigment cells
|Kuroda T,Yasuda S,Kusakawa S,Hirata N,Kanda Y,Suzuki K,Takahashi M,Nishikawa S,Kawamata S,Sato Y||PloS one (7:null)||2012|
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19.
MA1-813 was used in western blot to investigate the effects of pyruvate and DMEM on the differentiation of retinal pigment epithelial cell line ARPE-19
|Ahmado A,Carr AJ,Vugler AA,Semo M,Gias C,Lawrence JM,Chen LL,Chen FK,Turowski P,da Cruz L,Coffey PJ||Investigative ophthalmology & visual science (52:7148)||2011|
Cellular Retinaldehyde-Binding Protein; cellular retinaldehyde-binding protein-1; Retinaldehyde-binding protein 1
3110056M11Rik; BOS_20120; CRALBP; RLBP1