|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant CRALBP.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||1 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.02 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-813 detects CRALBP protein in bovine, human, mouse, non-human primate and rat samples.
MA1-813 has successfully been used in Western blot, immunohistochemistry, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects a 37 kDa protein representing human recombinant CRALBP.
The MA1-813 immunogen is purified human recombinant CRALBP.
Cellular retinaldehyde-binding protein (CRALBP) plays an important role in the regeneration of 11-cis-retinal for use in rod visual pigments such as opsin and rhodopsin. Once 11-cis-retinal is photoisomerized in the rod outer segment, it is converted to all-trans-retinal and further modified into all-trans-retinol. All-trans-retinol then diffuses into the retinal pigment epithelium (RPE) to be converted back to 11-cis-retinol and further oxidized into 11-cis-retinal (both by CRALBP). Genetic mutations involving CRALBP and quote;s lack of function have been linked to visual disease such as bothnia dystrophy, retinitis punctata albescens, retina pigmentosa, and Newfoundland rod-cone dystrophy. The presence of CRALBP serves as a marker for RPE and Muller glial cells of the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Microphthalmia-associated transcription factor regulates the visual cycle genes Rlbp1 and Rdh5 in the retinal pigment epithelium.
MA1-813 was used in western blot to investigate regulation of the visual cycle genes Rdh5 and Rlbp1 in the retinal pigment epithelium by microphthalmia-associated transcription factor
|Wen B,Li S,Li H,Chen Y,Ma X,Wang J,Lu F,Qu J,Hou L||Scientific reports (6:null)||2016|
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19.
MA1-813 was used in western blot to investigate the effects of pyruvate and DMEM on the differentiation of retinal pigment epithelial cell line ARPE-19
|Ahmado A,Carr AJ,Vugler AA,Semo M,Gias C,Lawrence JM,Chen LL,Chen FK,Turowski P,da Cruz L,Coffey PJ||Investigative ophthalmology and visual science (52:7148)||2011|
SOCS3 in retinal neurons and glial cells suppresses VEGF signaling to prevent pathological neovascular growth.
MA1-813 was used in immunohistochemistry to study the contribution of suppressor of cytokine signaling 3 to neovascularization
|Sun Y,Ju M,Lin Z,Fredrick TW,Evans LP,Tian KT,Saba NJ,Morss PC,Pu WT,Chen J,Stahl A,Joyal JS,Smith LE||Science signaling (8:null)||2015|
AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice.
MA1-813 was used in immunohistochemistry - paraffin section to explore gene therapy options to treat retinitis pigmentosa
|Choi VW,Bigelow CE,McGee TL,Gujar AN,Li H,Hanks SM,Vrouvlianis J,Maker M,Leehy B,Zhang Y,Aranda J,Bounoutas G,Demirs JT,Yang J,Ornberg R,Wang Y,Martin W,Stout KR,Argentieri G,Grosenstein P,Diaz D,Turner O,Jaffee BD,Police SR,Dryja TP||Molecular therapy. Methods and clinical development (2:null)||2015|
A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells.
MA1-813 was used in immunocytochemistry to determine the molecular mechanisms that affect the epithelial phenotype.
|Choudhary P,Dodsworth BT,Sidders B,Gutteridge A,Michaelides C,Duckworth JK,Whiting PJ,Benn CL||PloS one (10:null)||2015|
Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle.
MA1-813 was used in immunocytochemistry and western blot to study induced pluripotent stem cell-derived retinal pigmented epithelial cells for visual cycle function and sutability for transplantation
|Muñiz A,Greene WA,Plamper ML,Choi JH,Johnson AJ,Tsin AT,Wang HC||Investigative ophthalmology and visual science (55:198)||2014|
GABA-mediated induction of early neuronal markers expression in postnatal rat progenitor cells in culture.
MA1-813 was used in immunocytochemistry to study the response to GABA-mediated signaling of retinal progenitors derived from Müller glia
|Ramírez M,Hernández-Montoya J,Sánchez-Serrano SL,Ordaz B,Ferraro S,Quintero H,Peña-Ortega F,Lamas M||Neuroscience (224:210)||2012|
Diabetes-induced peroxynitrite impairs the balance of pro-nerve growth factor and nerve growth factor, and causes neurovascular injury.
MA1-813 was used in immunocytochemistry to investigate the mechanism for neurovascular dysfunction induced by oxidative stress
|Ali TK,Al-Gayyar MM,Matragoon S,Pillai BA,Abdelsaid MA,Nussbaum JJ,El-Remessy AB||Diabetologia (54:657)||2011|
Genetic deletion of COX-2 diminishes VEGF production in mouse retinal Müller cells.
MA1-813 was used in immunocytochemistry to investigate the role of COX2 in vascular endothelial growth factor production in retinal Muller cells
|Yanni SE,McCollum GW,Penn JS||Experimental eye research (91:34)||2010|
Loss of Müller's cells and photoreceptors in macular telangiectasia type 2.
MA1-813 was used in immunohistochemistry to report on the clinicopathology of a patient with macular telangiectasia type 2
|Powner MB,Gillies MC,Zhu M,Vevis K,Hunyor AP,Fruttiger M||Ophthalmology (120:2344)||2013|
Ghrelin modulates physiologic and pathologic retinal angiogenesis through GHSR-1a.
MA1-813 was used in immunohistochemistry to investigate the role of ghrelin in retinal vasculariztaion
|Zaniolo K,Sapieha P,Shao Z,Stahl A,Zhu T,Tremblay S,Picard E,Madaan A,Blais M,Lachapelle P,Mancini J,Hardy P,Smith LE,Ong H,Chemtob S||Investigative ophthalmology and visual science (52:5376)||2011|
Perifoveal müller cell depletion in a case of macular telangiectasia type 2.
MA1-813 was used in immunohistochemistry to report a clinical case of macular telangiectasia type 2 with perifoveal m
|Powner MB,Gillies MC,Tretiach M,Scott A,Guymer RH,Hageman GS,Fruttiger M||Ophthalmology (117:2407)||2010|
Neuroprotection in the juvenile rat model of light-induced retinopathy: evidence suggesting a role for FGF-2 and CNTF.
MA1-813 was used in immunohistochemistry to investigate the role of FGF-2 and CNTF in protecting the juvenile retina from light-induced damage
|Joly S,Pernet V,Chemtob S,Di Polo A,Lachapelle P||Investigative ophthalmology and visual science (48:2311)||2007|
Temporal/spatial expression of retinoid binding proteins and RAR isoforms in the postnatal lung.
MA1-813 was used in immunohistochemistry to study components of the retinoid-signaling pathway during postnatal alveologenesis in the mouse
|Hind M,Corcoran J,Maden M||American journal of physiology. Lung cellular and molecular physiology (282:L468)||2002|
Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells.
MA1-813 was used in flow cytometry to develop in vitro methods for the highly sensitive detection of residual undifferentiated induced pluripotent stem cells following differentiation to retinal pigment cells
|Kuroda T,Yasuda S,Kusakawa S,Hirata N,Kanda Y,Suzuki K,Takahashi M,Nishikawa S,Kawamata S,Sato Y||PloS one (7:null)||2012|