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Immunofluorescent analysis of CREB in HeLa cells using a CREB recombinant rabbit oligoclonal antibody (Product # 710149) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) and actin staining using Alexa Fluor 594 phalloidin (red) showing nuclear localization of CREB (Image A). Image B shows DAPI staining only (blue) and Image C is a composite image of an And B.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 222–341 of human CREB|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
CREB (cAMP responsive element binding protein) encodes for a 43 kDa protein transcription factor and belongs to leucine zipper family. CREB binds to the cyclic-AMP response element (CRE, a sequence present in many viral and cellular promoters) as a homodimer. Phosphorylated form of CREB act as an inducer of transcription for the genes, upon hormone dependent stimulation of cyclic-AMP pathway. CREB is known to play important role in circadian rythm generation and memory formation; aberration in CREB-mediated gene expression has been linked with Alzheimer disease. In humans, the gene is located on the q arm of chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, transactivator protein, Active transcription factor CREB, cAMP responsive element binding protein 1, CREB1, MGC9284, Transactivator protein