Western blot analysis of CRIP2 in E coli-derived fusion protein. Samples were probed with a CRIP2 polyclonal antibody (Product # PA1-20848) at a dilution of 1:2000 and a Goat anti-IgY-HRP secondary antibody at a dilution of 1:1000. Colorimetric method was use for signal development.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Fusion protein full length (Human).|
|Purification||Antigen affinity chromatography|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20848 detects CRIP2 in Human, Mouse and Rat samples.
PA1-20848 has been successfully used in Immunofluorescence and Western Blot procedures.
PA1-20848 immunogen corresponds to Fusion protein full length (Human)..
CRIP2 is a 208-amino acid protein containing 2 LIM domains and shares 93% amino acid sequence identity with its rat homolog, called Esp1 or Crp2. Northern blot analysis showed widespread tissue expression of the 1.3-kb CRIP2 mRNA, with the highest level of expression found in heart. In testis, a second 1.7-kb mRNA was also detected.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.