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Invitrogen
CRM1 Recombinant Rabbit Monoclonal Antibody (8H4L7)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
CRM1 Antibody (703238) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), SW480 (Lane 3), U-2 OS (Lane 4), PC-3 (Lane 5), A375 (Lane 6) and A549 (Lane 7). The blot was probed with Anti-CRM1 Recombinant Rabbit Monoclonal Antibody (Product # 703238, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~123 kDa band corresponding to CRM1 was observed in all the cell lines tested.
Product Details
703238
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H4L7
Immunogen
Protein corresponding to human Exportin 1 [aa824-aa1071]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784570
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig.^M
^M
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Exportin - 1 (XPO1) mediates the nuclear export of cellular proteins bearing a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, in association with RANBP3, XPO1 binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex is mediated through binding to nucleoproteins. Upon transit of a nuclear complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the cargo from the export receptor. The directionality of the nuclear export is thought to be conferred by an asymmetric distribution of the GTP and GDP bound forms of Ran between the cytoplasm and nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chromosome region maintenance 1 homolog; Chromosome region maintenance 1 protein homolog; Exp1; exportin 1 (CRM1 homolog, yeast); Exportin-1; exportin-1 (required for chromosome region maintenance)
Gene Aliases: CRM1; emb; exp1; XPO1
UniProt ID: (Human) O14980
Entrez Gene ID: (Human) 7514
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
negative regulation of transcription from RNA polymerase II promoter
protein export from nucleus
sister chromatid cohesion
regulation of centrosome duplication
viral process
protein localization to nucleus
regulation of protein catabolic process
response to drug
regulation of mRNA stability
regulation of protein export from nucleus
mRNA transport
intracellular transport of virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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