Western blot of CRX/Cone-rod Homeobox in rat retina lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a CRX/Cone-rod Homeobox polyclonal antibody (Product# PA5-37855) at a dilution of 1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-DPLDYKDQSAWK, from the internal region near the C-terminus of CRX|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human, mouse, pig and cow based on sequence homology.
The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.