|Immunohistochemistry (Paraffin) (IHC (P))||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15383 targets CRYAB in IHC and WB applications and shows reactivity with Human samples.
The MA5-15383 immunogen is purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander and quote;s disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; CRYAB; crystallin, alpha B; CTPP2; epididymis secretory protein Li 101; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: CMD1II; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
UniProt ID: (Human) P02511
Entrez Gene ID: (Human) 1410
Molecular Function: chaperone
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