Abnova

CSK Monoclonal Antibody (3A3)

View all (28) CSK antibodies

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Questions & Answers

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CSK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration is 1 µg/mL.

CSK Antibody in Western Blot (WB) — Western blot analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi (Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

CSK Antibody in Western Blot (WB) — Western Blot analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.

Lane 1: CSK transfected lysate(50.7 KDa).
Lane 2: Non-transfected lysate.

CSK Antibody in Western Blot (WB) — CSK monoclonal antibody (M01), clone 3A3. Western Blot analysis of CSK expression in HeLa S3 NE (Cat # L013V3).

CSK Antibody in Western Blot (WB) — CSK monoclonal antibody (M01), clone 3A3 Western Blot analysis of CSK expression in HL-60 (Cat # L014V1).

CSK Antibody in Immunoprecipitation (IP) — Immunoprecipitation of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CSK MaxPab rabbit polyclonal antibody.

CSK Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged CSK is approximately 0.1 ng/mL as a capture antibody.

Product Details

H00001445-M01

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1 µg/mL	-
ELISA (ELISA)	0.1 ng/mL	-
Immunoprecipitation (IP)	1-5 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	3A3
Immunogen	CSK (NP_004374, 1 a.a. approximately 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	See Label
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE

Target Information

C-terminal Src kinase (CSK) is a non-receptor protein tyrosine kinase that plays an important role in the regulation of cell growth, differentiation, migration, and immune responses. CSK phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases including LCK, SRC, HCK, FYN, LYN, CSK, and YES1. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-Src kinase; MGC117393; Protein-tyrosine kinase CYL; Tyrosine-protein kinase CSK

Gene Aliases: CSK

UniProt ID: (Human) P41240

Entrez Gene ID: (Human) 1445

regulation of cytokine production adaptive immune response protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway central nervous system development brain development negative regulation of cell proliferation negative regulation of low-density lipoprotein particle clearance cell migration cell differentiation T cell costimulation negative regulation of interleukin-6 production negative regulation of kinase activity adherens junction organization peptidyl-tyrosine autophosphorylation regulation of cell proliferation negative regulation of Golgi to plasma membrane protein transport positive regulation of MAP kinase activity innate immune response negative regulation of bone resorption oligodendrocyte differentiation negative regulation of phagocytosis T cell receptor signaling pathway regulation of Fc receptor mediated stimulatory signaling pathway negative regulation of ERK1 and ERK2 cascade cellular response to peptide hormone stimulus

Process(es)

protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding protein C-terminus binding kinase activity protein phosphatase binding protein kinase A catalytic subunit binding identical protein binding metal ion binding proline-rich region binding