Western blot analysis of Casein kinase 1 e using anti-Casein kinase 1 e monoclonal antibody (MA5-17231) on (Lane 1) HeLa Cell lysate, (Lane 2) Ramos Cell lysate, (Lane 3) A431 Cell lysate, (Lane 4) 293T Cell lysate, (Lane 5) Jurket Cell lysate and (Lane 6) PC12 Cell lysate.
|Tested species reactivity||Mouse, Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli (His-Casein Kinase Ie)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||1 ul/400 ul lysate|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is 293T cells.
Casein kinases are a group of ubiquitous Ser/Thr kinases that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, circadian rhythms, and metabolic pathways. CK1 family members share a highly conserved kinase domain but differ in their variable N-and C-terminal domains. Mammals have seven family members: alpha, beta, gamma1, gamma2, gamma3, delta and epsilon. They all share at least 50% amino acid identity within the protein kinase catalytic domain. CK1 isoforms exclusively use ATP as phosphate donor and are generally co-factor independent. Casein kinase I epsilon is a Wnt-regulated kinase, and regulated phosphorylation of Dvl allows fine tuning of the Wnt-b-catenin signaling pathway. CKIe positively regulates Akt possibly by inhibiting PP2A. CKIe play an important role in neurodegenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.