Western blot analysis of CTB and CTG1 in 20µg of NIH-3T3 lysates using a CTB and CTG1 polyclonal antibody (Product # PA1-32416) at a dilution of 1:15,000 (lane 1), 1:10,000 dilution (lane 2) and 1:15,000 dilution (lane 3) followed by ECL detection at an exposure time of 30 seconds.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope that this antibody recognizes maps to the N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709. The N-terminus of beta actin is highly conserved with gamma actin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris phosphate/tris citrate, pH 7|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000-1:15,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32416 detects CTB and CTG1 from human, mouse samples.
PA1-32416 has been successfully used in Western blot applications.
The PA1-32416 immunogen is: The epitope that this antibody recognizes maps to the N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709. The N-terminus of beta actin is highly conserved with gamma actin.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.