|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human CTCF expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15792 targets CTCF in WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-15792 immunogen is purified recombinant fragment of human CTCF expressed in E. Coli.
MA5-15792 detects CTCF which has a predicted molecular weight of approximately 83kDa.
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.Tissue specificity: Ubiquitous. Absent in primary spermatocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.