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CTCFL Antibody (MA5-49545) in WB
Western blot analysis of CTCFL in mouse testis tissue lysate (15 µg). Samples were incubated with CTCFL monoclonal antibody (Product # MA5-49545) with a dilution of 1 µg/mL, followed by peroxidase conjugated goat anti-rat IgG secondary antibody. CTCFL band was visualized using ECL Western Blotting Substrate. Result: MA5-49545 can detect mouse CTCFL by Western blotting.
Product Details
MA5-49545
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KT72
Immunogen
Recombinant Mouse Ctcfl
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3090705
Product Specific Information
This product was used in an Indirect ELISA.
Target Information
CTCFL is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes. CTCFL is normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BORIS; BORIS like protein; Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF T; CTCF-like protein; HMG 1L1; likely orthologue of H. sapiens CCCTC-binding factor-like protein (CTCFL); MGC163358; MGC169105; MGC169106; Transcriptional repressor CTCFL
Gene Aliases: Boris; Ctcfl; OTTMUSG00000016680
UniProt ID: (Mouse) A2APF3
Entrez Gene ID: (Mouse) 664799
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
DNA binding
protein binding
histone binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
C2H2 zinc finger transcription factor
regulation of gene expression by genetic imprinting
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of gene expression
chromatin modification
histone methylation
DNA methylation involved in gamete generation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of histone H3-K4 methylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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