Invitrogen

CTLA-4 Monoclonal Antibody (1B8), FITC

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CTLA-4 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CTLA-4 in Con-A and IL-2 stimulated BALB/c mouse splenocytes. Cells were stained with CTLA-4 Monoclonal Antibody (1B8), FITC (Product # HMCD15201).

CTLA-4 Antibody in ELISA (ELISA) — ELISA analysis of CTLA-4. The ELISA plate was coated with mouse CD152/CTLA-4 and purified/unlabeled Rat IgG1 kappa. CTLA-4 and purified immunoglobulin were detected with serially diluted CTLA-4 Monoclonal Antibody (1B8), FITC (Product # HMCD15201).

Product Details

HMCD15201

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.1-0.2 µg / 1x10^6 cells	View 3 publications 3 publications
ELISA (ELISA)	3 µg/10^6 cells	-
Neutralization (Neu)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Armenian hamster / IgG1
Class	Monoclonal
Type	Antibody
Clone	1B8
Immunogen	Mouse CD152 (CTLA-4). from Extracellular portion of murine CTLA-4 fused to a murine IgG2a
Conjugate	FITC PE
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	purified
Storage buffer	PBS
Contains	<0.1% sodium azide
Storage conditions	4° C, store in dark
RRID	AB_2536593

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer.

Therapeutic advances in medical oncology

Doo DW,Meza-Perez S,Londoño AI,Goldsberry WN,Katre AA,Boone JD,Moore DJ,Hudson CT,Betella I,McCaw TR,Gangrade A,Bao R,Luke JJ,Yang ES,Birrer MJ,Starenki D,Cooper SJ,Buchsbaum DJ,Norian LA,Randall TD,Arend RC

HMCD15201 was used in Flow cytometry/Cell sorting to determine whether WNT974 would impair tumor growth, affect gene expression patterns, and improve the immune response in human and murine ovarian cancer models.

Thu Apr 14 00:00:00 EDT 2022

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Journal for immunotherapy of cancer

Hu J,He J,Wang Y,Zhao Y,Fang K,Dong Y,Chen Y,Zhang Y,Zhang C,Wang H,Tan J,Wang J,Zi R,Liu C,Liang H,Guo Y,Ou J

HMCD15201 was used in Flow Cytometry to provide a promising strategy for sensitizing poorly immunogenic solid tumors to immunotherapy in the clinic.

Tue Mar 01 00:00:00 EST 2022

Inhibiting WNT Ligand Production for Improved Immune Recognition in the Ovarian Tumor Microenvironment.

Cancers

Goldsberry WN,Meza-Perez S,Londoño AI,Katre AA,Mott BT,Roane BM,Goel N,Wall JA,Cooper SJ,Norian LA,Randall TD,Birrer MJ,Arend RC

HMCD15201 was used in Flow cytometry/Cell sorting to study Wnt activity within the microenvironment of ovarian tumors.

Tue Mar 24 00:00:00 EDT 2020

Enhancement of antitumor immunity by CTLA-4 blockade.

Science (New York, N.Y.)

Leach DR,Krummel MF,Allison JP

HMCD15201 was used in blocking or activating experiment to show that administration f antibodies that recognize CTLA-4 results in rejection of tumors

Fri Mar 22 00:00:00 EST 1996

Bioinformatics

Protein Aliases: CD152; CD152 antigen; CTLA-4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; ICOS

Gene Aliases: Cd152; Ctla-4; Ctla4; Ly-56

UniProt ID: (Mouse) P09793

Entrez Gene ID: (Mouse) 12477

Molecular Function: immunoglobulin receptor superfamily

Function(s)

immune system process immune response cellular response to DNA damage stimulus negative regulation of B cell proliferation negative regulation of T cell proliferation positive regulation of apoptotic process negative regulation of regulatory T cell differentiation negative regulation of immune response B cell receptor signaling pathway

CTLA-4 Monoclonal Antibody (1B8), FITC

Product Details

HMCD15201

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

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