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Immunofluorescent analysis of CUGBP2 showing staining in the nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CUGBP2 polyclonal antibody (Product # PA5-34856) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contains a sequence corresponding to a region within amino acids 282 and 508 (O95319) of CUGBP2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34856 targets CUGBP2 in IHC (P) and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34856 immunogen is recombinant protein fragment contains a sequence corresponding to a region within amino acids 282 and 508 (O95319) of CUGBP2.
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
bruno-like protein 3; BRUNOL3; CELF 2; CELF2; CUG triplet repeat RNA-binding protein 2; CUG triplet repeat, RNA binding protein 2; CUG-BP- and ETR-3-like factor 2; CUGBP Elav-like family member 2; CUGBP2; ELAV-type RNA-binding protein 3; ETR-3; ETR3; mNapor; NAPOR; neuroblastoma apoptosis-related RNA-binding protein; RNA-binding protein BRUNOL-3
B230218O03; B230345P09Rik; BRUNOL3; C88023; CELF-2; CELF2; CUG-BP2; CUGBP2; D230046B21Rik; ETR-3; ETR3; mETR-3; NAPOR; Napor-2