|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||2-3 µg/ml|
|Western Blot (WB)||1:250-1:1000|
|Western Blot (WB)||See 9 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Insect , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminus of the human CUL2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
CUL-2 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5), homologous to the S. cerevisiae cdc53 gene. CUL-1 forms a complex with human p19skp1 and F box protein p45skp2. Together with cdc53 (Known as CulA), as three subunits, they form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL-1, CUL-2 does not associate with p19skp1 and p45skp2. Instead, CUL-2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CUL-2; Cullin-2; testis secretory sperm-binding protein Li 238E
Gene Aliases: 1300003D18Rik; 4932411N15Rik; AI327301; CUL2; mKIAA4106
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.