Western blot analysis of CUL-3 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human CUL3-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CUL-3 polyclonal antibody (Product # PA5-21511) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 352 and 656 of Cullin 3 (Uniprot ID#Q13618)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21511 targets CUL-3 in Western blot applications and shows reactivity with Human samples.
The PA5-21511 immunogen is a recombinant fragment corresponding to a region within amino acids 352 and 656 of Cullin 3 (Uniprot ID#Q13618).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.