Invitrogen

CXCL1 Monoclonal Antibody (48415)

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated. View Details

View all (17) CXCL1 (GRO alpha) antibodies

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Datasheet

Questions & Answers

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CXCL1 Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouse CXCL1/GROa/KC/CINC-1 aa 20-96 chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Product # MA5-23745). The ND50 is typically 0.05-0.25 µg/mL.

CXCL1 Antibody in Neutralization — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CXCL1 is shown by the decrease in fluorescence (Chemotaxis measured by Resazurin) with increasing concentrations of CXCL1 Antibody (Product # MA5-23745). {Neu}

Product Details

Tested Applications

Dilution

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

Neutralization (Neu)

0.05-0.25 µg/mL

Western Blot (WB)

1 µg/mL

Product Specifications

Species Reactivity

Mouse

Host / Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

48415

Immunogen

E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1 Arg20-Lys96

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2609463

Product Specific Information

This antibody shows 100% cross-reactivity with recombinant human (rh) CXCL2, less than 40% cross-reactivity with recombinant rat (rr) CINC-1, and no cross-reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse (rm) CXCL6.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

KC, a homolog of human and hamster gro/MGSA, is a 72 amino acid CXC chemokine originally cloned from rat macrophages and lung tissue. It is the mediator for recruitment and activation of neutrophils in rat lung inflammation models. Expression of KC can be upregulated by LPS and IL-1b stimulation. IFN-gamma blocks LPS induced expression of KC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha-chemokine; C-X-C motif chemokine 1; CXCL-1; CXCL1; Fsp; gro; Gro1; GRO1 oncogene; Growth-regulated alpha protein; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; Mgsa; N51; Platelet-derived growth factor-inducible protein KC; Scyb1; Secretory protein N51

Gene Aliases: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

UniProt ID: (Mouse) P12850

Entrez Gene ID: (Mouse) 14825

Molecular Function: chemokine cytokine signaling molecule