Invitrogen

CXCL1 Monoclonal Antibody (48415)

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

3 Published Figures

2 References

View all (24) CXCL1 (GRO alpha) antibodies

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CXCL1 Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouse CXCL1/GROa/KC/CINC-1 aa 20-96 chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Product # MA5-23745). The ND50 is typically 0.05-0.25 µg/mL.

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CXCL1 is shown by the decrease in fluorescence (Chemotaxis measured by Resazurin) with increasing concentrations of CXCL1 Antibody (Product # MA5-23745). {Neu}

CXCL1 Antibody in Immunohistochemistry (IHC) — Figure 2 FALCs Are Covered by a Differentiated Monolayer of Mesothelial Cells (A) 3D reconstruction of a large portion of the omentum obtained by imaging of wholemount staining of the omentum showing omFALCs with DAPI (white). (B and C) Confocal imaging and 3D reconstruction of an omFALC showing a view of the surface of the cluster and a z section (along the dotted line) of the cluster (B) and of the surface of the omentum (C) with PDPN (red), CXCL13 (green), DAPI (blue), and IgM (yellow). (D-F) Representative confocal imaging and 3D reconstruction of an omFALC showing a view of the surface of the cluster and a z section (dotted line) of the cluster with PDPN (red); ENPP2 (D, green), Cathepsin-C (E, CTSC, green), or ISG15 (F, green); DAPI (blue) and IgM (D, yellow); or CD45 (E and F, yellow). The surface of the cluster and the mesothelium are delimited by a hyphenated line. (G) Violin plots of gene expression of inflammatory chemokines by cluster with highest log-normalized expression value labeled. (H) Amounts of CCL2, CXCL10, and CXCL1 secreted into the supernatant of 2-h omentum explant culture per omentum and per mL after exposure to Zymosan-A for 2 h either in vivo (i.p. injection) or ex vivo . Data pooled from two independent experiments with n = 10 mice per group. (I) Confocal imaging and 3D reconstruction of omFALC showing the surface of omFALCs (first column) and a clipped view inside the cluster (second column). Scale bars, 100 mum. All staining representative of n

CXCL1 Antibody in Flow Cytometry (Flow) — Figure 3 CXCL1 Is Required for the Recruitment of Neutrophils into omFALCs (A) Number of neutrophils in digested omentum as assessed by flow-cytometric analysis ( Figure S5 A) of naive (white bar) and at the indicated time points following i.p. injection of Zymosan-A (blue bars). Data pooled from two independent experiments with n = 5-11 mice per group. (B) Representative confocal imaging and 3D reconstruction of omFALCs from naive and 6 h post-Zymosan mice, DAPI (magenta) and Ly6G (white). Scale bar, 200 mum. (C) Quantification of the mean gray value of Ly6G and MPO stained as in Figure S5 B of omenta from naive (white bar) and at the indicated time points following i.p. injection of Zymosan (blue bars). Data for cluster quantification pooled from two independent experiments with n >= 24 imaged clusters from n = 8 mice per group. (D) Quantification of the volume of omFALCs at the indicated times following i.p. injection of Zymosan-A (blue). Data pooled from two independent experiments with n >= 32 imaged clusters from n = 6 mice per group. (E) Representative confocal images of omentum, the peritoneal surface of the diaphragm, and parietal wall from naive and 6 h post-Zymosan mice, DAPI (magenta) and Ly6G (white) and quantification of the mean gray value of Ly6G for omFALCs, parietal wall, diaphragm from naive (white) and 6 h post-Zymosan mice (blue). Data for cluster quantification pooled from two independent experiments with n >= 24 imaged clusters from n = 6 mice per group

Product Details

MA5-23745

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)	-
Neutralization (Neu)	0.05-0.25 µg/mL	-

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	48415
Immunogen	E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1 Arg20-Lys96 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.5 mg/mL
Purification	Protein A/G
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2609463

Product Specific Information

This antibody shows 100% cross-reactivity with recombinant human (rh) CXCL2, less than 40% cross-reactivity with recombinant rat (rr) CINC-1, and no cross-reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse (rm) CXCL6.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants.

Immunity

Jackson-Jones LH,Smith P,Portman JR,Magalhaes MS,Mylonas KJ,Vermeren MM,Nixon M,Henderson BEP,Dobie R,Vermeren S,Denby L,Henderson NC,Mole DJ,Bénézech C

MA5-23745 was used in Immunohistochemistry-immunofluorescence to show how specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.

Tue Apr 14 00:00:00 EDT 2020

Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants.

Immunity

Jackson-Jones LH,Smith P,Portman JR,Magalhaes MS,Mylonas KJ,Vermeren MM,Nixon M,Henderson BEP,Dobie R,Vermeren S,Denby L,Henderson NC,Mole DJ,Bénézech C

Tue Apr 14 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; CXCL-1; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

Gene Aliases: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

UniProt ID: (Mouse) P12850

Entrez Gene ID: (Mouse) 14825

Molecular Function: chemokine