Invitrogen
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1 µg/mL | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
- | View 1 publication 1 publication |
ELISA (ELISA) |
0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture) | - |
Neutralization (Neu) |
0.05-0.25 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Rat / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
48415 |
Immunogen |
E. coli-derived recombinant mouse CXCL1/GRO alpha/KC/CINC-1 Arg20-Lys96 |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
0.5 mg/mL |
Purification |
Protein A/G |
Storage buffer |
PBS with 5% trehalose |
Contains |
No Preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2609463 |
This antibody shows 100% cross-reactivity with recombinant human (rh) CXCL2, less than 40% cross-reactivity with recombinant rat (rr) CINC-1, and no cross-reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse (rm) CXCL6.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; CXCL-1; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51
Gene Aliases: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1
UniProt ID: (Mouse) P12850
Entrez Gene ID: (Mouse) 14825
Molecular Function:
chemokine
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