Western blot analysis of human ITAC was performed by loading 0.25 µg of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with an anti -human ITAC monoclonal antibody (Product # MA1032:clone 10C6, MA1033: clone 22F1 ) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length recombinant protein expressed in E coli.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.125 - 0.25 µg/ml|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
The Molecular weight is ~8303 Da from positions 22 - 94 as determined by MALDI.
Interferon-inducible T-cell alpha chemoattractant ( I-TAC), belonging to the CXC chemokine family, is expressed in peripheral blood leukocytes, pancreas and liver, thymus, spleen, lung, small intestine, placenta and prostate. Its molecular weight is 8–11 kD. I-TAC is also known as CXCL-11 and IP-9. The cytokine is involved in regulating calcium release and inducing a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. It is upregulated in patients with AIDS, dementia and in patients with multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.