|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human IL-8.|
|Storage buffer||PBS, pH 7.5|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 9 publications below|
This product is recommended as a capture antibody in Sandwich ELISA applications.
Interleukin 8 is a pro-inflammatory factor which belongs to the CXC subfamily of chemokinesk, and which are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and is a potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.
AHC0982 was used in ELISA to report that SFKs regulate the chemokine induction activity of LL-37 in monocytic cells.
|Nijnik A,Pistolic J,Cho P,Filewod NC,Falsafi R,Ramin A,Harder KW,Hancock RE||Journal of leukocyte biology (91:599)||2012|
Rheumatoid factor and its interference with cytokine measurements: problems and solutions.
AHC0982 was used in ELISA to study cytokine levels in patients with rheumatoid arthritis and determine the effect of interference of rheumatoid factor.
|Bartels EM,Falbe Wätjen I,Littrup Andersen E,Danneskiold-Samsøe B,Bliddal H,Ribel-Madsen S||Arthritis (2011:null)||2011|
IL-8 and cathepsin B as melanoma serum biomarkers.
AHC0982 was used in ELISA to study IL8 and Cathepsin B in the prognosis of melanoma mortality.
|Zhang H,Fu T,McGettigan S,Kumar S,Liu S,Speicher D,Schuchter L,Xu X||International journal of molecular sciences (12:1505)||2011|
|Not Applicable||Not Cited||
Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.
AHC0982 was used in ELISA to optimize innate defense regulator derivatives with enhanced chemokine induction
|Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE||Journal of immunology (Baltimore, Md. : 1950) (184:2539)||2010|
Igf-I inhibits spontaneous apoptosis in human granulocytes.
AHC0982 was used in ELISA to examine the effects of IGF-I on in vitro apoptosis of human peripheral blood granulocytes.
|Kooijman R,Coppens A,Hooghe-Peters E||Endocrinology (143:1206)||2002|
|Not Applicable||Not Cited||
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
AHC0982 was used in ELISA to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors
|Suwa T,Hogg JC,Vincent R,Mukae H,Fujii T,van Eeden SF||Experimental lung research (28:1)||2002|
|Mouse||Not Cited||Biological characterization of lipopolysaccharide from Treponema pectinovorum.||Kesavalu L,Falk CW,Davis KJ,Steffen MJ,Xu X,Holt SC,Ebersole JL||Infection and immunity (70:211)||2002|
|Human||Not Cited||Parathyroid hormone-related protein induces interleukin 8 production by prostate cancer cells via a novel intracrine mechanism not mediated by its classical nuclear localization sequence.||Gujral A,Burton DW,Terkeltaub R,Deftos LJ||Cancer research (61:2282)||2001|
|Human||Not Cited||Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.||Nixon CS,Steffen MJ,Ebersole JL||Infection and immunity (68:5284)||2000|