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Immunofluorescence analysis of CXCL9 / MIG was performed using 70% confluent log phase THP-1 cells treated with 100ng of IFN-gamma for 15 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ABfinity™ CXCL9 / MIG (11H1L14) Recombinant Rabbit Monoclonal Antibody (701117) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 23–125 of human monokine induced by Interferon-gamma|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||0.1-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
MIG belongs to a family of chemoattractant cytokines that play a role in monocyte, and T lymphocyte chemoattraction. MIG is expressed in monocytes/macrophages, hepatocytes, fibroblasts, keratinocytes, and endothelial cells in response to interferon-gamma. MIG is related functionally to interferon-inducible protein 10 (IP10), as both bind to the CXCR3 receptor to inhibit endothelial cell chemotaxis, and growth factor-induced angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
chemokine (C-X-C motif) ligand 9; CMK; crg-10; gamma-interferon-induced monokine; Humig; MIG; monokine induced by gamma interferon; monokine induced by interferon-gamma; SCYB9; small-inducible cytokine B9
CMK; crg-10; CXCL9; Humig; MIG; SCYB9