|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues 1-19 of human CXCR2.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is human isolated neutrophils.
CXCR1 and CXCR2, previously known as IL-8RA, or type I IL-8 receptor, and IL-8RB, or type II IL-8 receptor, respectively, have been shown to share approximately 77% amino acid sequence identity. IL-8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca2+ levels. While IL-8 and Granulocyte Chemotactic Protein 2 (GCP2) have been shown to exert their biological activity by binding to both receptors, GROa, GROß, GRO?, NAP-2 and ENA-78 bind only to CXCR2. CXCR1 and CXCR2 are expressed on neutrophils and mast cells, but not on B or T lymphocytes. In cells transfected with either CXCR1 or CXCR2, clone 19 showed no cross-reactivity with CXCR1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal transduction Cell surface receptor mediated signal transduction G-protein mediated signaling Immunity and defense Granulocyte-mediated immunity Cytokine/chemokine mediated immunity Cell structure and motility Cell motility