|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||SIV-infected SupT1 cells injected into Balb/c mice|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Analyte Specific Reagent
Multisystemic Disease Modeling of Liver-Derived Protein Folding Disorders Using Induced Pluripotent Stem Cells (iPSCs).
MHCXCR404 was used in immunohistochemistry to describe protocols to directed differentiation of protein folding disease-specific iPSCs into hepatocytes and neural-lineage cells.
|Leung A,Murphy GJ||Methods in molecular biology (Clifton, N.J.) (1353:261)||2015|
Co-culture of hematopoietic stem cells with mesenchymal stem cells increases VCAM-1-dependent migration of primitive hematopoietic stem cells.
MHCXCR404 was used in flow cytometry to investigate how mesenchymal stem cells improve hematopoietic stem cell transplantability.
|Perdomo-Arciniegas AM,Vernot JP||International journal of hematology (94:525)||2011|
KPNA2 expression is an independent adverse predictor of biochemical recurrence after radical prostatectomy.
MHCXCR404 was used in immunohistochemistry - paraffin section to examine karyopherin alpha 2 expression in prostate cancer samples.
|Mortezavi A,Hermanns T,Seifert HH,Baumgartner MK,Provenzano M,Sulser T,Burger M,Montani M,Ikenberg K,Hofstädter F,Hartmann A,Jaggi R,Moch H,Kristiansen G,Wild PJ||Clinical cancer research : an official journal of the American Association for Cancer Research (17:1111)||2011|