|Tested species reactivity||Human, Mouse|
|Published species reactivity||Hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 182-196 of CXCR4 receptor.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control for this product is HeLa cell lysate.
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to T-cell antigen T4 (CD4), to infect target cells. Some G protein-coupled receptors including chemokine (C-C) receptor 5 (CCR5), chemokine (C-X-C) receptor 4 (CXCR4), CCR3, CCR2b, and CCR8 in the chemokine receptor family, and four new human molecules G-protein coupled receptor 15 (GPR15), CXCR6, GPR1, and chemokine (C-X3-C) receptor 1 (CX3CR1) were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR, or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The alpha-chemokine stromal cell-derived factor-1 (SDF-1) is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlate with HIV-1 permissiveness in diverse human cell types. Some antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation of Toll-like receptor 3 amplifies mesenchymal stem cell trophic factors and enhances therapeutic potency.
PA1-12542 was used in western blot to study the effect of TLR-3 activation on mesenchymal stem cell trophic factors and therapeutic potency
|Mastri M,Shah Z,McLaughlin T,Greene CJ,Baum L,Suzuki G,Lee T||American journal of physiology. Cell physiology (303:C1021)||2012|