|Flow Cytometry (Flow)||Assay-dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-dependent|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in octyl glucoside)|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is specific for human Cytochrome b245 light chain (aa 165-169, KKPSE).
This target has a predicted molecular weight of 21 kDa.
Cytochrome b is composed of cytochrome b alpha and beta chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease. In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cytochrome b light chain; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; Cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit
Gene Aliases: CYBA; p22-PHOX
UniProt ID: (Human) P13498
Entrez Gene ID: (Human) 1535
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