Invitrogen

CYLD Polyclonal Antibody

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CYLD Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of CYLD in paraformaldehyde-fixed A431 cells using CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:200.

CYLD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of CYLD was performed in paraffin-embedded human gastric cancer tissue using CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

CYLD Antibody in Western Blot (WB) — Western Blot analysis of CYLD was performed by separating 30 µg of various whole cell lysates by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. C8D30, B. NIH-3T3 , C. BCL-1 , D. Raw264.7 , E. C2C12.

Product Details

PA5-34630

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region within amino acids 896 and 956 of CYLD View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2551982

Product Specific Information

Recommended positive controls: HepG2, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.

Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cylindromatosis Is Required for Survival of a Subset of Melanoma Cells.

Oncology research

La T,Jin L,Liu XY,Song ZH,Farrelly M,Feng YC,Yan XG,Zhang YY,Thorne RF,Zhang XD,Teng L

PA5-34630 was used in Immunohistochemistry to reveal an unexpected role of CYLD in promoting survival of a subset of melanoma cells and uncover the heterogeneity of CYLD expression and its biological significance in melanoma.

Tue Sep 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: cylindromatosis (turban tumor syndrome); Deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; retinitis pigmentosa 1 homolog; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD

Gene Aliases: 2010013M14Rik; 2900009M21Rik; BRSS; C130039D01Rik; CDMT; CYLD; CYLD1; CYLDI; EAC; HSPC057; KIAA0849; LRRGT00003; MFT; MFT1; mKIAA0849; Rp1; Rp1h; SBS; TEM; USPL2

UniProt ID: (Human) O94934, (Rat) Q66H62, (Mouse) Q80TQ2

Entrez Gene ID: (Human) 1540, (Rat) 312937, (Mouse) 74256

Molecular Function: ribosomal protein