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Invitrogen
CYP1A1/CYP1A2 Monoclonal Antibody (MC1)
FIGURE: 1 / 7
CYP1A1/CYP1A2 Antibody (MA3-036) in ICC/IF
Immunofluorescent analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) in 3% BSA-PBS at a dilution of 1:100 and incu... View More
Product Details
MA3-036
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MC1
Immunogen
3-methylcholanthrene induced rat cytochrome P450 protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_1073970
Product Specific Information
MA3-036 detects Cytochrome P450 1A1/1A2 from human, primate, rat and mouse samples.
MA3-036 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunohistochemisty (paraffin) applications. By Western blot, this antibody detects a ~58 kDa band representing Cytochrome P450 1A1/1A2.
The MA3-036 immunogen is 3-methylcholanthrene induced rat cytochrome P450 protein.
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aromatic compound inducible; aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CYP1A1; CYPIA1; CYPIA2; Cytochrome P(3)450; Cytochrome P-448; Cytochrome P-450d; cytochrome P1-450; cytochrome P1-450, dioxin-inducible; Cytochrome P3 450; Cytochrome P450 1A1; Cytochrome P450 1A2; Cytochrome P450 4; cytochrome P450 family 1 subfamily a polypeptide 1; cytochrome P450 family 1 subfamily a polypeptide 2; Cytochrome P450 form 6; cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c); Cytochrome P450 subfamily I (aromatic compound-inducible) member A2 (Q42 form d); cytochrome P450 subfamily I, polypeptide 1; cytochrome P450, 1a1; cytochrome P450, 1a1, aromatic compound inducible; cytochrome P450, 1a2; cytochrome P450, 1a2, aromatic compound inducible; cytochrome P450, family 1, subfamily A, polypeptide 1; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), member A1 (C6, form c) (C6, form c); cytochrome P450, subfamily I (aromatic compound-inducible), member A2 (Q42, form d); cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; Cytochrome P450-C; Cytochrome P450-D; Cytochrome P450-P1; Cytochrome P450-P2; Cytochrome P450-P3; Cytochrome P450MT2; Cytochrome P450MT2A; Cytochrome P450MT2B; dioxin-inducible; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P-448; P450 form 4; P450-D; P450form6; P450MT2; xenobiotic monooxygenase
Gene Aliases: AHH; AHRR; CP11; CP12; CYP1; Cyp1a-1; Cyp1a-2; CYP1A1; CYP1A2; Cyp45c; Cypc45c; CYPD45; CYPIA1; P-450d; P-450MC; P1-450; P3-450; P450(PA); P450-1; P450-3; P450-C; P450DX; RATCYPD45
UniProt ID: (Human) P04798, (Human) P05177, (Mouse) P00184, (Rat) P00185, (Mouse) P00186, (Rat) P04799
Entrez Gene ID: (Human) 1543, (Human) 1544, (Mouse) 13076, (Rat) 24296, (Mouse) 13077, (Rat) 24297
Molecular Function:
oxidoreductase
oxygenase
metabolite interconversion enzyme
response to hypoxia
porphyrin-containing compound metabolic process
aging
cell proliferation
amine metabolic process
response to wounding
response to virus
response to nematode
response to herbicide
ethylene metabolic process
embryo development ending in birth or egg hatching
coumarin metabolic process
flavonoid metabolic process
response to iron(III) ion
insecticide metabolic process
drug metabolic process
dibenzo-p-dioxin catabolic process
epoxygenase P450 pathway
response to food
response to lipopolysaccharide
response to vitamin A
response to immobilization stress
vitamin D metabolic process
response to drug
9-cis-retinoic acid biosynthetic process
camera-type eye development
response to antibiotic
response to arsenic-containing substance
digestive tract development
hydrogen peroxide biosynthetic process
response to hyperoxia
oxidation-reduction process
maternal process involved in parturition
hepatocyte differentiation
demethylation
cellular response to organic cyclic compound
omega-hydroxylase P450 pathway
positive regulation of G1/S transition of mitotic cell cycle
steroid catabolic process
xenobiotic metabolic process
toxin biosynthetic process
post-embryonic development
alkaloid metabolic process
regulation of gene expression
monoterpenoid metabolic process
dibenzo-p-dioxin metabolic process
lung development
methylation
response to estradiol
monocarboxylic acid metabolic process
drug catabolic process
exogenous drug catabolic process
cellular respiration
heterocycle metabolic process
oxidative demethylation
cellular response to cadmium ion
oxidative deethylation
toxin metabolic process
response to toxic substance
liver development
response to organic substance
response to organic cyclic compound
developmental process
cellular aromatic compound metabolic process
monooxygenase activity
iron ion binding
protein binding
steroid hydroxylase activity
oxidoreductase activity
oxidoreductase activity, acting on diphenols and related substances as donors
flavonoid 3'-monooxygenase activity
oxygen binding
enzyme binding
heme binding
demethylase activity
aromatase activity
vitamin D 24-hydroxylase activity
electron carrier activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
caffeine oxidase activity
catalytic activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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