MA3-032 detects Cytochrome P450 3A4/3A5 from human cell line HepG2, and from bovine liver and pancreas samples.
MA3-032 has been successfully used in Western blot applications. By Western blot, this antibody detects a ~54 kDa band representing Cytochrome P450 3A4/3A5.
The MA3-032 immunogen is a synthetic peptide corresponding to residues E S R D G T V S G A, conjugated to ovalbumin.
This gene,CYP3A5, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. This cluster includes a pseudogene, CYP3A5P1, which is very similar to CYP3A5. This similarity has caused some difficulty in determining whether cloned sequences represent the gene or the pseudogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CP35; CYP3A4; CYP3A5; CYPIIIA28; CYPIIIA3; CYPIIIA4; CYPIIIA5; cytochrome P450 3A28; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 3A5; Cytochrome P450 HLp; Cytochrome P450 HLp2; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; cytochrome P450, subfamily IIIA, polypeptide 4; Cytochrome P450-PCN1; Cytochrome P450-PCN3; flavoprotein-linked monooxygenase; glucocorticoid-inducible P450; microsomal monooxygenase; Nifedipine oxidase; P450-III, steroid inducible; P450PCN3; PCN3; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase; xenobiotic monooxygenase
Gene Aliases: BOS_22940; BOS_22960; CP33; CP34; CP35; CYP3A; CYP3A28; CYP3A3; CYP3A4; CYP3A5; CYPIIIA3; CYPIIIA4; CYPIIIA5; HLP; NF-25; P450C3; P450PCN1; P450PCN3; PCN3