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Western blot analysis was performed on whole cell extracts (30 ug lysate) of Rat Brain (Lane 1), and Mouse Brain (Lane 2). The blots were probed with Anti-CaM Kinase II Mouse Monoclonal Antibody (Product# MA1048, 0.5-1 ug/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A ~ 50 kDa band corresponding to CaM Kinase II was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified, rat CaM kinase II.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.5-1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-048 detects CaM kinase II from rat and mouse tissues. This antibody has been shown to detect both the phosphorylated and non-phosphorylated alpha subunit of CaM kinase II only. MA1-048 does not detect the ~60 kDa beta subunit in either phosphorylation state.
MA1-048 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing the alpha subunit of CaM kinase II. Immunofluorescence staining of CaM kinase II in rat hippocampal cells with MA1-048 results in intense staining of cell somas and neuropil areas, while nuclei are only lightly stained.
The MA1-048 immunogen is partially purified, rat CaM kinase II.
CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region.
Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Direct visualization of newly synthesized target proteins in situ.
MA1-048 was used in western blot to describe and use a method to visualize newly synthesized proteins specifically.
|tom Dieck S,Kochen L,Hanus C,Heumüller M,Bartnik I,Nassim-Assir B,Merk K,Mosler T,Garg S,Bunse S,Tirrell DA,Schuman EM||Nature methods (12:411)||2015|
Activated CaMKII couples GluN2B and casein kinase 2 to control synaptic NMDA receptors.
MA1-048 was used in western blot to study the control of synaptic NMDA receptors by CaMKII, GluN2B and CK2
|Sanz-Clemente A,Gray JA,Ogilvie KA,Nicoll RA,Roche KW||Cell reports (3:607)||2013|
Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT).
MA1-048 was used in western blot to study the modulation of the hippocampal neuropil proteome by a D1/D5 receptor agonist
|Hodas JJ,Nehring A,Höche N,Sweredoski MJ,Pielot R,Hess S,Tirrell DA,Dieterich DC,Schuman EM||Proteomics (12:2464)||2012|
Attention deficit/hyperactivity disorder-derived coding variation in the dopamine transporter disrupts microdomain targeting and trafficking regulation.
MA1-048 was used in western blot to study the role of the ADHD-derived coding variation in the dopamine transporter in disrupting microdomain targeting and trafficking
|Sakrikar D,Mazei-Robison MS,Mergy MA,Richtand NW,Han Q,Hamilton PJ,Bowton E,Galli A,Veenstra-Vanderweele J,Gill M,Blakely RD||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:5385)||2012|
Testosterone exerts antiapoptotic effects against H2O2 in C2C12 skeletal muscle cells through the apoptotic intrinsic pathway.
MA1-048 was used in western blot to study the antiapoptotic effect of testosterone
|Pronsato L,Boland R,Milanesi L||The Journal of endocrinology (212:371)||2012|
GluN2B subunit deletion reveals key role in acute and chronic ethanol sensitivity of glutamate synapses in bed nucleus of the stria terminalis.
MA1-048 was used in western blot to study the role of GluN2B subunit in ethanol sensitivity
|Wills TA,Klug JR,Silberman Y,Baucum AJ,Weitlauf C,Colbran RJ,Delpire E,Winder DG||Proceedings of the National Academy of Sciences of the United States of America (109:E278)||2012|
Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation.
MA1-048 was used in western blot to characterize a CaMKII-binding densin domain capable of regulating glutamate receptor phosphorylation
|Jiao Y,Jalan-Sakrikar N,Robison AJ,Baucum AJ,Bass MA,Colbran RJ||The Journal of biological chemistry (286:24806)||2011|
Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.
MA1-048 was used in western blot to identify the spinophilin interactome in rodent striatum
|Baucum AJ,Jalan-Sakrikar N,Jiao Y,Gustin RM,Carmody LC,Tabb DL,Ham AJ,Colbran RJ||Molecular & cellular proteomics : MCP (9:1243)||2010|
Dysregulation of dopamine transporters via dopamine D2 autoreceptors triggers anomalous dopamine efflux associated with attention-deficit hyperactivity disorder.
MA1-048 was used in western blot to investigate the molecular mechanism for the attention-deficit hyperactivity disorder
|Bowton E,Saunders C,Erreger K,Sakrikar D,Matthies HJ,Sen N,Jessen T,Colbran RJ,Caron MG,Javitch JA,Blakely RD,Galli A||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:6048)||2010|
Loss of GluN2B-containing NMDA receptors in CA1 hippocampus and cortex impairs long-term depression, reduces dendritic spine density, and disrupts learning.
MA1-048 was used in western blot to investigate the role of GluN2B for long-term depression, dendritic spine density, and memory formation
|Brigman JL,Wright T,Talani G,Prasad-Mulcare S,Jinde S,Seabold GK,Mathur P,Davis MI,Bock R,Gustin RM,Colbran RJ,Alvarez VA,Nakazawa K,Delpire E,Lovinger DM,Holmes A||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:4590)||2010|
Genetic disruption of the alternative splicing of drebrin gene impairs context-dependent fear learning in adulthood.
MA1-048 was used in western blot to compare the role of drebrin in neuronal function and cognitive process
|Kojima N,Hanamura K,Yamazaki H,Ikeda T,Itohara S,Shirao T||Neuroscience (165:138)||2010|
Object-place recognition learning triggers rapid induction of plasticity-related immediate early genes and synaptic proteins in the rat dentate gyrus.
MA1-048 was used in western blot to investigate expression of immediate early genes related to the plasticity in the dentate gyrus following long-term object-place recognition learning in rats
|Soulé J,Penke Z,Kanhema T,Alme MN,Laroche S,Bramham CR||Neural plasticity (2008:null)||2009|
Calmodulin kinase II is involved in voltage-dependent facilitation of the L-type Cav1.2 calcium channel: Identification of the phosphorylation sites.
MA1-048 was used in western blot to investigate the mechanism for calcium-dependent facilitation of L-type calcium channels involving the phosphorylation status of calmodulin kinase II
|Lee TS,Karl R,Moosmang S,Lenhardt P,Klugbauer N,Hofmann F,Kleppisch T,Welling A||The Journal of biological chemistry (281:25560)||2006|
Selective brain responses to acute and chronic low-dose X-ray irradiation in males and females.
MA1-048 was used in western blot to study the differential brain responses to low-dose X-ray irradiation between sexes
|Silasi G,Diaz-Heijtz R,Besplug J,Rodriguez-Juarez R,Titov V,Kolb B,Kovalchuk O||Biochemical and biophysical research communications (325:1223)||2004|
Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II.
MA1-048 was used in western blot to study the CPEB phosphorylation by LTP-activated CaMKII, CaMKIV, PKA, and protein kinase C .
|Atkins CM,Nozaki N,Shigeri Y,Soderling TR||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:5193)||2004|
Brief seizure activity alters Ca2+/calmodulin dependent protein kinase II dephosphorylation and subcellular distribution in rat brain for several hours.
MA1-048 was used in western blot to investigate the effect of brief seizure activity on calcium/calmodulin dependent protein kinase II dephosphorylation and subcellular distribution in rat brain
|Dong Y,Rosenberg HC||Neuroscience letters (357:95)||2004|
Prolonged changes in Ca2+/calmodulin-dependent protein kinase II after a brief pentylenetetrazol seizure; potential role in kindling.
MA1-048 was used in western blot to investigate the role CaMKII in PTZ kindling
|Dong Y,Rosenberg HC||Epilepsy research (58:107)||2004|
Eye opening induces a rapid dendritic localization of PSD-95 in central visual neurons.
MA1-048 was used in western blot to investigate the redistribution of PSD-95 in central visual neurons induced by eye opening.
|Yoshii A,Sheng MH,Constantine-Paton M||Proceedings of the National Academy of Sciences of the United States of America (100:1334)||2003|
The cardiac-specific nuclear delta(B) isoform of Ca2+/calmodulin-dependent protein kinase II induces hypertrophy and dilated cardiomyopathy associated with increased protein phosphatase 2A activity.
MA1-048 was used in western blot to investigate the role of CaMKII in the development of the hypertrophy and dilated cardiomyopathy.
|Zhang T,Johnson EN,Gu Y,Morissette MR,Sah VP,Gigena MS,Belke DD,Dillmann WH,Rogers TB,Schulman H,Ross J,Brown JH||The Journal of biological chemistry (277:1261)||2002|
The septin CDCrel-1 is dispensable for normal development and neurotransmitter release.
MA1-048 was used in western blot to study the non-essential function of CDCrel-1 during neuronal development or function.
|Peng XR,Jia Z,Zhang Y,Ware J,Trimble WS||Molecular and cellular biology (22:378)||2002|
Tetanic stimulation leads to increased accumulation of Ca(2+)/calmodulin-dependent protein kinase II via dendritic protein synthesis in hippocampal neurons.
MA1-048 was used in western blot to show that the tetanic stimulation can cause an increase in the concentration of alpha-CaMKII in the dendrites of postsynaptic neurons
|Ouyang Y,Rosenstein A,Kreiman G,Schuman EM,Kennedy MB||The Journal of neuroscience : the official journal of the Society for Neuroscience (19:7823)||1999|
Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta.
MA1-048 was used in western blot to compare the ligand binding specificity and distribution between estrogen receptor alpha and beta
|Kuiper GG,Carlsson B,Grandien K,Enmark E,Häggblad J,Nilsson S,Gustafsson JA||Endocrinology (138:863)||1997|
Ablation of ErbB4 from excitatory neurons leads to reduced dendritic spine density in mouse prefrontal cortex.
MA1-048 was used in immunohistochemistry to study the effects on murine prefrontal cortex dendritic spines of whole-brain and excitatory neuron-specific ablation of ErbB4
|Cooper MA,Koleske AJ||The Journal of comparative neurology (522:3351)||2014|
Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats.
MA1-048 was used in immunohistochemistry to study stress-induced reinstatement of palatable food seeking by female rats and the role of the dorsal medial prefrontal cortex
|Calu DJ,Kawa AB,Marchant NJ,Navarre BM,Henderson MJ,Chen B,Yau HJ,Bossert JM,Schoenbaum G,Deisseroth K,Harvey BK,Hope BT,Shaham Y||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:214)||2013|
Role of orbitofrontal cortex neuronal ensembles in the expression of incubation of heroin craving.
MA1-048 was used in immunohistochemistry to study the role of orbitalfrontal cortex assemblies in the enhanced response to heroin cues following heroin withdrawal in rats
|Fanous S,Goldart EM,Theberge FR,Bossert JM,Shaham Y,Hope BT||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:11600)||2012|
Sustained Arc/Arg3.1 synthesis controls long-term potentiation consolidation through regulation of local actin polymerization in the dentate gyrus in vivo.
MA1-048 was used in immunohistochemistry to investigate the role of the activity-regulated cytoskeletal-associated protein (Arc) and activity-regulated gene 3.1 protein homolog (Arg3.1) in long-term potentiation .
|Messaoudi E,Kanhema T,Soulé J,Tiron A,Dagyte G,da Silva B,Bramham CR||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:10445)||2007|
Differential expression of PSD proteins in age-related spatial learning impairments.
MA1-048 was used in immunohistochemistry to study age-induced changes in postsynaptic density protein expression in spatial learning impairments
|Nyffeler M,Zhang WN,Feldon J,Knuesel I||Neurobiology of aging (28:143)||2007|
Roles of glutamate receptors and the mammalian target of rapamycin (mTOR) signaling pathway in activity-dependent dendritic protein synthesis in hippocampal neurons.
MA1-048 was used in immunohistochemistry to study the roles of glutamate receptors and the mTOR signaling pathway in the dendritic protein synthesis in hippocampal neurons.
|Gong R,Park CS,Abbassi NR,Tang SJ||The Journal of biological chemistry (281:18802)||2006|
Non-nuclear estrogen receptor beta and alpha in the hippocampus of male and female rats.
MA1-048 was used in immunohistochemistry to investigate the expression and subcellular localization of estrogen receptor beta in rat hippocampus
|Kalita K,Szymczak S,Kaczmarek L||Hippocampus (15:404)||2005|
Altered prelimbic cortex output during cue-elicited drug seeking.
MA1-048 was used in immunohistochemistry to investigate the effect of cocaine on the shift of prefrontal cortex cell population.
|Miller CA,Marshall JF||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:6889)||2004|
Dlx1 transcription factor regulates dendritic growth and postsynaptic differentiation through inhibition of neuropilin-2 and PAK3 expression.
MA1-048 was used in immunocytochemistry to study the roles of neurophilin-2 and PAK3 in the mechanism by which Dlx1 modulates the growth and post-synaptic differentiation of dendritic cells
|Dai X,Iwasaki H,Watanabe M,Okabe S||The European journal of neuroscience (39:531)||2014|
Calmodulin kinase II regulates the maturation and antigen presentation of human dendritic cells.
MA1-048 was used in immunocytochemistry to investigate the role of calmodulin kinase II (CamK II) for the maturation and antigen presentation of human dendritic cells
|Herrmann TL,Morita CT,Lee K,Kusner DJ||Journal of leukocyte biology (78:1397)||2005|
Synapse composition and organization following chronic activity blockade in cultured hippocampal neurons.
MA1-048 was used in immunocytochemistry to study the effect of activity on synapse composition and organization in central nerve system
|Harms KJ,Craig AM||The Journal of comparative neurology (490:72)||2005|
NMDA receptor activation mediates copper homeostasis in hippocampal neurons.
MA1-048 was used in immunocytochemistry to study the mechanisms for copper homeostasis in hippocampal neurons.
|Schlief ML,Craig AM,Gitlin JD||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:239)||2005|
alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; Calcium/calmodulin-dependent protein kinase type 2; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II subunit alpha; caM kinase II subunit alpha; caM-kinase II alpha chain; CaMK II; caMK-II subunit alpha; CaMKII
Camk2a; CaMKII; mKIAA0968; PK2CDD; PKCCD; R74975