Western blot analysis was performed on tissue extracts (30 µg lysate) of Rat Brain (Lane 1), and Mouse Brain (Lane 2). The blots were probed with Anti-CaM Kinase II alpha Mouse Monoclonal Antibody (Product# 13-7300, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjµgate (Product # 626520, 1:4000 dilution). A ~ 54 kDa band corresponding to CaM Kinase II alpha was observed across tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Sheep, Rat, Fish|
|Published species reactivity||Rat, Hamster, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Purified rat brain CaM Kinase.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1 µg/mL|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-5 µg/ml|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||0.5-1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for alpha subunit of CaM Kinase II. No significant reactivity was seen with the B, B1, yB, dA, dB, and dC isoforms. The epitope is located in the regulatory domain.
Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD andreduces LTP and learning (Elgersma et al., 2002
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Evolutionary and functional perspectives on signaling from neuronal surface to nucleus.
13-7300 was used in immunocytochemistry to review the mechanism of a gammaCaMKII-CaM nuclear translocation.
|Cohen SM,Li B,Tsien RW,Ma H||Biochemical and biophysical research communications (460:88)||2015|
miR-26a and miR-384-5p are required for LTP maintenance and spine enlargement.
13-7300 was used in immunocytochemistry to study the roles of miR-26a and miR-384-5p in long-term potentiation
|Gu QH,Yu D,Hu Z,Liu X,Yang Y,Luo Y,Zhu J,Li Z||Nature communications (6:null)||2015|
|Hamster||Not Cited||Netrin-1 induces axon branching in developing cortical neurons by frequency-dependent calcium signaling pathways.||Tang F,Kalil K||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:6702)||2005|
Melatonin stimulates dendrite formation and complexity in the hilar zone of the rat hippocampus: participation of the Ca++/Calmodulin complex.
13-7300 was used in western blot to study the effect of melatonin on dendrite formation in the hilar zone of the rat hippocampus
|Domínguez-Alonso A,Valdés-Tovar M,Solís-Chagoyán H,Benítez-King G||International journal of molecular sciences (16:1907)||2015|
The schizophrenia susceptibility gene dysbindin regulates dendritic spine dynamics.
13-7300 was used in immunocytochemistry and western blot to study the role of dysbindin in the regulation of dendritic spine dynamics
|Jia JM,Hu Z,Nordman J,Li Z||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:13725)||2014|
|Mouse||Not Cited||Calcium and calmodulin-dependent serine/threonine protein kinase type II (CaMKII)-mediated intramolecular opening of integrin cytoplasmic domain-associated protein-1 (ICAP-1¿) negatively regulates ß1 integrins.||Millon-Frémillon A,Brunner M,Abed N,Collomb E,Ribba AS,Block MR,Albigès-Rizo C,Bouvard D||The Journal of biological chemistry (288:20248)||2013|
Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis.
13-7300 was used in western blot to investigate how BDNF selectively affects mRNA expression.
|Huang YW,Ruiz CR,Eyler EC,Lin K,Meffert MK||Cell (148:933)||2012|
Stimulation-mediated translocation of calmodulin and neurogranin from soma to dendrites of mouse hippocampal CA1 pyramidal neurons.
13-7300 was used in western blot to investigate the association of calmodulin and neurogranin in neurons.
|Huang KP,Huang FL,Shetty PK||Neuroscience (178:1)||2011|
Delayed treatment with a novel neurotrophic compound reduces behavioral deficits in rabbit ischemic stroke.
13-7300 was used in western blot to study the efficacy and mechanism of CNB-00 in a rabbit ischemic stroke model.
|Lapchak PA,Schubert DR,Maher PA||Journal of neurochemistry (116:122)||2011|
|Rat||Not Cited||Calcium-calmodulin-dependent protein kinase II contributes to spinal cord central sensitization.||Fang L,Wu J,Lin Q,Willis WD||The Journal of neuroscience : the official journal of the Society for Neuroscience (22:4196)||2002|
|Human||1:500||PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.||Christopherson KS,Hillier BJ,Lim WA,Bredt DS||The Journal of biological chemistry (274:27467)||1999|
||Calcium and calmodulin-dependent serine/threonine protein kinase type II (CaMKII)-mediated intramolecular opening of integrin cytoplasmic domain-associated protein-1 (ICAP-1¿) negatively regulates ß1 integrins.||Millon-Frémillon A,Brunner M,Abed N,Collomb E,Ribba AS,Block MR,Albigès-Rizo C,Bouvard D||The Journal of biological chemistry (288:20248)||2013|
||Calcium-calmodulin-dependent protein kinase II contributes to spinal cord central sensitization.||Fang L,Wu J,Lin Q,Willis WD||The Journal of neuroscience : the official journal of the Society for Neuroscience (22:4196)||2002|