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Western blot analysis was performed on whole cell extracts (30 ug lysate) of SH-SY5Y (Lane 1), U-87MG (Lane 2) and PC12 (Lane 3). The blots were probed with Anti-CaM Kinase II beta Mouse Monoclonal Antibody (Product# 139800, 0.5-1 ug/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A ~ 70 kDa band corresponding to CaM Kinase II beta was observed only in PC12. As it is a Rat immunogen, it did not react with human cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Fish, Human, Mouse, Sheep, Rat, Sea urchin|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Purified rat brain CaM Kinase.|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||0.5 - 1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities.
13-9800 was used in western blot to characterize rat cerebellar, hippocampal and cortical postsynaptic densities
|Farley MM,Swulius MT,Waxham MN||Neuroscience (304:286)||2015|
Targeting the CaMKII/ERK Interaction in the Heart Prevents Cardiac Hypertrophy.
13-9800 was used in western blot to test if CaMKII association promotes cardiac hypertrophy through ERK nuclear localization.
|Cipolletta E,Rusciano MR,Maione AS,Santulli G,Sorriento D,Del Giudice C,Ciccarelli M,Franco A,Crola C,Campiglia P,Sala M,Gomez-Monterrey I,De Luca N,Trimarco B,Iaccarino G,Illario M||PloS one (10:null)||2015|
|Mouse||Not Cited||Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.||Elgersma Y,Fedorov NB,Ikonen S,Choi ES,Elgersma M,Carvalho OM,Giese KP,Silva AJ||Neuron (36:493)||2002|
|Human||1:500||PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.||Christopherson KS,Hillier BJ,Lim WA,Bredt DS||The Journal of biological chemistry (274:27467)||1999|
Evolutionary and functional perspectives on signaling from neuronal surface to nucleus.
13-9800 was used in immunocytochemistry to review the mechanism of a gammaCaMKII-CaM nuclear translocation.
|Cohen SM,Li B,Tsien RW,Ma H||Biochemical and biophysical research communications (460:88)||2015|
Structure and composition of the postsynaptic density during development.
13-9800 was used in immunocytochemistry and western blot to study the structure and composition of post-synaptic densities during development
|Swulius MT,Kubota Y,Forest A,Waxham MN||The Journal of comparative neurology (518:4243)||2010|
Electron cryotomography of postsynaptic densities during development reveals a mechanism of assembly.
13-9800 was used in immunohistochemistry to elucidate the 3D structure of postsynaptic densities.
|Swulius MT,Farley MM,Bryant MA,Waxham MN||Neuroscience (212:19)||2012|
Loss of GluN2B-containing NMDA receptors in CA1 hippocampus and cortex impairs long-term depression, reduces dendritic spine density, and disrupts learning.
13-9800 was used in western blot to characterize mice lacking GluN2B in the pyramidal neurons of the cortex and CA1 subregion of the hippocampus
|Brigman JL,Wright T,Talani G,Prasad-Mulcare S,Jinde S,Seabold GK,Mathur P,Davis MI,Bock R,Gustin RM,Colbran RJ,Alvarez VA,Nakazawa K,Delpire E,Lovinger DM,Holmes A||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:4590)||2010|
Ca; Ca++/calmodulin-dependent protein kinase II beta subunit; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II beta subunit; caM kinase II subunit beta; CaM-kinase II beta chain; CaMK II; caMK-II subunit beta; proline rich calmodulin-dependent protein kinase
CAM2; CAMK2; CAMK2B; Camk2d; CAMKB; Ck2b