|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human CaM kinase kinase alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is brain tissue.
Ca2+/Calmodulin dependent protein kinases (CaM Kinase I, II and IV) are considered to play a central role in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP), receptor function, modification of the cytoskeleton and regulation of gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.