Immunofluorescent staining of PC12 cells using PA5-19661, anti-CaMKI gamma antibody. The cells were fixed with PFA (4%) for 10 minutes,permabilised with TBS-T (20mins), BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
This antibody is predicted to react with mouse, cow and human based on sequence homology.
This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Evolutionary and functional perspectives on signaling from neuronal surface to nucleus.
PA5-19661 was used in immunocytochemistry to review the mechanism of a gammaCaMKII-CaM nuclear translocation.
|Cohen SM,Li B,Tsien RW,Ma H||Biochemical and biophysical research communications (460:88)||2015|