MA1-048 detects CaM kinase II from rat and mouse tissues. This antibody has been shown to detect both the phosphorylated and non-phosphorylated alpha subunit of CaM kinase II only. MA1-048 does not detect the ~60 kDa beta subunit in either phosphorylation state.
MA1-048 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing the alpha subunit of CaM kinase II. Immunofluorescence staining of CaM kinase II in rat hippocampal cells with MA1-048 results in intense staining of cell somas and neuropil areas, while nuclei are only lightly stained.
The MA1-048 immunogen is partially purified, rat CaM kinase II.
CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region.
Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.
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Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; Calcium/calmodulin-dependent protein kinase type 2; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; caM-kinase II alpha chain; CaMK II; caMK-II subunit alpha; CaMKII
Gene Aliases: Camk2a; CaMKII; mKIAA0968; PK2CDD; PKCCD; R74975
Molecular Function: non-receptor serine/threonine protein kinase