Immunofluorescence analysis of Pan-Cadherin was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cadherin pan Rabbit Polyclonal Antibody (717100) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Chicken, Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Dog, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the carboxy-terminus of the chicken N-cadherin protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2.5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This peptide antibody is broadly cross-reactive with all members of the cadherin family of proteins including N-cadherin, E-cadherin, P-cadherin, and R-cadherin. The antibody also displays broad species cross-reactivity including human, bovine, mouse, rat, chicken, amphibian, as well as other species. Rabbit anti-pan-Cadherin is useful as both a ubiquitous cadherin probe as well as a marker for adherens junctions.
71-7100 was used successfully in the immunofluorescence analysis of pan cadherin in MDCK cells.
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
71-7100 was used in immunocytochemistry and western blot to study the differentiation and transformation of canine kidney MDCK cells cultured in either a 2D or 3D environment
|Töyli M,Rosberg-Kulha L,Capra J,Vuoristo J,Eskelinen S||Laboratory investigation; a journal of technical methods and pathology (90:915)||2010|
Immunofluorescent analysis of connexin-43 using monoclonal antibodies to its extracellular domain.
71-7100 was used in immunohistochemistry - frozen section to assess the use of connexin-43 monoclonal antibodies
|Baklaushev VP,Gurina OI,Yusubalieva GM,Grinenko NF,Cytrin EB,Victorov IV,Chekhonin VP||Bulletin of experimental biology and medicine (148:725)||2009|
Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose.
71-7100 was used in western blot to investigate the effects of glucose and shear stress using bovine aortic endothelial cells
|Wang XL,Fu A,Spiro C,Lee HC||Journal of proteomics and bioinformatics (2:null)||2009|
|Human||Not Cited||Differential regulation of the TRAIL death receptors DR4 and DR5 by the signal recognition particle.||Ren YG,Wagner KW,Knee DA,Aza-Blanc P,Nasoff M,Deveraux QL||Molecular biology of the cell (15:5064)||2004|
|Human||Not Cited||Myofibroblast development is characterized by specific cell-cell adherens junctions.||Hinz B,Pittet P,Smith-Clerc J,Chaponnier C,Meister JJ||Molecular biology of the cell (15:4310)||2004|
p27Kip1 is expressed in proliferating cells in its form phosphorylated on threonine 187.
71-7100 was used in immunohistochemistry - paraffin section and western blot to demonstrate that p27 is expressed in proliferating cells
|Troncone G,Martinez JC,Iaccarino A,Zeppa P,Caleo A,Russo M,Migliaccio I,Motti ML,Califano D,Palmieri EA,Palombini L||BMC clinical pathology (5:null)||2005|
|Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis.||Croft DR,Sahai E,Mavria G,Li S,Tsai J,Lee WM,Marshall CJ,Olson MF||Cancer research (64:8994)||2004|
|Human||1:400||ESX induces transformation and functional epithelial to mesenchymal transition in MCF-12A mammary epithelial cells.||Schedin PJ,Eckel-Mahan KL,McDaniel SM,Prescott JD,Brodsky KS,Tentler JJ,Gutierrez-Hartmann A||Oncogene (23:1766)||2004|