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|Tested species reactivity||Mouse , Human , Rat , Cattle|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||2 ug/ml|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-934 detects human, bovine, mouse, and rat calcium sensing receptor.
MA1-934 has been successfully used in Western blot, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~ 150 and 130 kDa proteins from human HEK-293 cells.This antibody has also been used in immunohistochemical on frozen bovine parathyroid gland and rat thyroid gland.
The MA1-934 immunogen is a synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.
The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nutrient sensing receptors in gastric endocrine cells.
MA1-934 was used in immunohistochemistry to evaluate GPRC6A and CaSR receptors as nutrient sensing receptors in gastric endocrine cells
|Haid D,Widmayer P,Breer H||Journal of molecular histology (42:355)||2011|
Rab1 small GTP-binding protein regulates cell surface trafficking of the human calcium-sensing receptor.
MA1-934 was used in western blot to investigate the mediation of the cell surface trafficking of hCaR by Rab1
|Zhuang X,Adipietro KA,Datta S,Northup JK,Ray K||Endocrinology (151:5114)||2010|
Elucidation of the role of peptide linker in calcium-sensing receptor activation process.
MA1-934 was used in western blot to study the role of the peptide linker in calcium-sensing receptor activation
|Ray K,Adipietro KA,Chen C,Northup JK||The Journal of biological chemistry (282:5310)||2007|
HHC1, EIG8, BOS_823, HHC, HYPOC1, CaR, Gprc2a, FHH, FIH, CAR, PCAR1, NSHPT, GPRC2A
extracellular calcium-sensing receptor, parathyroid Ca(2+)-sensing receptor 1, parathyroid cell calcium-sensing receptor, G protein coupled receptor, family C, group 2, member A, G protein-coupled receptor, family C, group 2, member A, PCaR1, cation sensing receptor, calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism), parathyroid Cell calcium-sensing receptor, Calcium-sensing receptor (hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism), Calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism)