Invitrogen

Caldesmon Recombinant Rabbit Monoclonal Antibody (SU03-19)

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Datasheet

Protocols

Questions & Answers

Tech Support

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Caldesmon Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Caldesmon in NIH/3T3 cells using a Caldesmon Monoclonal antibody (Product # MA5-32107) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Caldesmon Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Caldesmon of paraffin-embedded Mouse kidney tissue using a Caldesmon Monoclonal antibody (Product #MA5-32107). Counter stained with hematoxylin.

Caldesmon Antibody in Western Blot (WB) — Western blot analysis of Caldesmon in NIH/3T3 cell lysate using a Caldesmon Monoclonal antibody (Product # MA5-32107) at a dilution of 1:1,000.

Caldesmon Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of Caldesmon in NIH/3T3 cells using a Caldesmon Monoclonal Antibody (Product # MA5-32107) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32107

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	SU03-19
Immunogen	Synthetic peptide within Human Caldesmon aa 744-793 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809400

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Caldesmon is a developmentally regulated protein involved in smooth muscle and non muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their electrophorectic mobility and cellular distribution. The h caldesmon variant (120 - 150 kDa) is predominantly expressed in smooth muscle whereas l caldesmon (70 - 80 kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Caldesmon; CDM; h-caldesmon; L-caldesmon; MGC21352; Non-muscle caldesmon; OTTHUMP00000207950; OTTHUMP00000207953; OTTHUMP00000207954; OTTHUMP00000207955; OTTHUMP00000214473; testis secretory sperm-binding protein Li 227n

Gene Aliases: 4833423D12Rik; AI195384; AV071549; AW536160; C920027I18Rik; CAD; CALD1; CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22

UniProt ID: (Human) Q05682, (Rat) Q62736

Entrez Gene ID: (Human) 800, (Rat) 25687, (Mouse) 109624

Molecular Function: non-motor actin binding protein