Immunofluorescent analysis of Caldesmon HMW (green) showing staining in human uterus tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Caldesmon HMW monoclonal antibody (Product # MA5-11626) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-488 conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with DAPI (blue) and images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Crude human uterus extract|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA5-11626 targets Caldesmon HMW in IHC (P), IF and WB applications and shows reactivity with human and mouse samples.
The MA5-11626 immunogen is crude human uterus extract.
Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TGF-beta1 drives partial myofibroblastic differentiation in chondromyxoid fibroma of bone.
MA5-11626 was used in immunohistochemistry to study the role of TGF-beta in myofibroblastic differentiation in chondromyxoid fibroma
|Romeo S,Eyden B,Prins FA,Briaire-de Bruijn IH,Taminiau AH,Hogendoorn PC||The Journal of pathology (208:26)||2006|
ALK-ATIC fusion in urinary bladder inflammatory myofibroblastic tumor.
MA5-11626 was used in immunohistochemistry to report molecular and clinicopathologic features of urinary bladder inflammatory myofibroblastic tumor
|Debiec-Rychter M,Marynen P,Hagemeijer A,Pauwels P||Genes, chromosomes and cancer (38:187)||2003|