Immunofluorescent analysis of Calmodulin using Calmodulin Monoclonal antibody (2D1) (Product# MA3-917) shows staining in HeLa cells. Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin (Product# MA3-917) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Bacteria, Bovine, Chicken, Human, Mouse, Rat|
|Published species reactivity||Fungi, Rat, Shrew, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Calmodulin purified from Dictyostelium discoideum.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||2µg per 10^6 cells|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-917 detects calmodulin from human, bovine, chicken, Chlamydomonas, Dictyostelium, mouse and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).
MA3-917 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-917 results in staining of the contractile vacuoles.
The MA3-917 antigen is calmodulin purified from Dictostelium discoideum.
Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Anti-Yo antibody uptake and interaction with its intracellular target antigen causes Purkinje cell death in rat cerebellar slice cultures: a possible mechanism for paraneoplastic cerebellar degeneration in humans with gynecological or breast cancers.
MA3-917 was used in blocking or activating experiment to investigate anit-Yo antibodies in Purkinje cell death
|Greenlee JE,Clawson SA,Hill KE,Wood B,Clardy SL,Tsunoda I,Carlson NG||PloS one (10:null)||2015|
Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.
MA3-917 was used in western blot to analyze Drosophila pericentrin and its requirement for interaction with calmodulin for function at centrosomes and neuronal basal bodies but not sperm basal bodies
|Galletta BJ,Guillen RX,Fagerstrom CJ,Brownlee CW,Lerit DA,Megraw TL,Rogers GC,Rusan NM||Molecular biology of the cell (25:2682)||2014|
Involvement of the plasma membrane Ca2+-ATPase in the short-term response of Arabidopsis thaliana cultured cells to oligogalacturonides.
MA3-917 was used in western blot to study the mechanism for the effect of oligogalacturonide on Arabidopsis thaliana cultured cells
|Romani G,Bonza MC,Filippini I,Cerana M,Beffagna N,De Michelis MI||Plant biology (Stuttgart, Germany) (6:192)||2004|
Characterization of the calcium-binding contractile protein centrin from Tetraselmis striata (Pleurastrophyceae).
MA3-917 was used in western blot to investigate the biochemical properties of centrin purified from Tetraselmis striata
|Coling DE,Salisbury JL||The Journal of protozoology (39:385)||1992|
Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva).
MA3-917 was used in immunohistochemistry to study the mechanism for serotonin 5-HT3 receptor-mediated vomiting
|Zhong W,Hutchinson TE,Chebolu S,Darmani NA||PloS one (9:null)||2014|
Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum.
MA3-917 was used in immunocytochemistry to investigate the interaction between calmodulin and the contractile vacuole complex during the mitosis of Dictyostelium discoideum
|Zhu Q,Liu T,Clarke M||Journal of cell science (104 ( Pt 4):1119)||1993|