Immunofluorescent analysis of calnexin (red) in U87 cells. Cell fixed with 4% paraformaldehyde were permeabilized with 0.05% Triton X-100 in PBS for 60 seconds at room temperature and blocked with 5% normal donkey serum for 30 minutes at room temperature. Cells were probed with a calnexin monoclonal antibody (Product # MA3-027) at a dilution of 1:100 for 2 hours at room temperature, washed with PBS, and incubated with a fluorescently-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:100 for 45 minutes at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a fluorescent microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Non-human primate, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Focus human hepatoma cell lysate.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-027 detects calnexin from human and mouse tissues.
MA3-027 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunoprecipitation protocols. By Western blot, this antibody detects an ~67 kDa band representing calnexin in human liver, skin, adrenal gland, heart, colon, testes and ovary extracts. This antibody is not recommended for human kidney or mouse liver lysates in Western blot applications. Immunocytochemical staining of calnexin in Huh-7 cells with MA3-027 yields a pattern consistent with specific endoplasmic reticulum staining.
The MA3-027 antigen is Focus human hepatoma cell lysate.
Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER.
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.
MA3-027 was used in immunocytochemistry to analyze control of MT1-MMP endocytic and E-cadherin polarized golgi trafficking to promote invasive breast cancer programs by RAB2A
|Kajiho H,Kajiho Y,Frittoli E,Confalonieri S,Bertalot G,Viale G,Di Fiore PP,Oldani A,Garre M,Beznoussenko GV,Palamidessi A,Vecchi M,Chavrier P,Perez F,Scita G||EMBO reports (17:1061)||2016|
The human IFN-inducible p53 target gene TRIM22 colocalizes with the centrosome independently of cell cycle phase.
MA3-027 was used in immunocytochemistry to investigate the colocalization of human IFN-inducible p53 target gene TRIM22 with the centrosome
|Petersson J,Lönnbro P,Herr AM,Mörgelin M,Gullberg U,Drott K||Experimental cell research (316:568)||2010|
Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis.
MA3-027 was used in immunocytochemistry to investigate the roles of Csp and Hdj-2 during the biosynthesis of immature CFTR.
|Zhang H,Schmidt BZ,Sun F,Condliffe SB,Butterworth MB,Youker RT,Brodsky JL,Aridor M,Frizzell RA||The Journal of biological chemistry (281:11312)||2006|
The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma.
MA3-027 was used in immunocytochemistry to demonstrate the role of the liver-enriched bZIP transcription factor CREB-H on hepatic physiology and hepatocarcinogenesis.
|Chin KT,Zhou HJ,Wong CM,Lee JM,Chan CP,Qiang BQ,Yuan JG,Ng IO,Jin DY||Nucleic acids research (33:1859)||2005|
The stop transfer sequence of the human UDP-glucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms.
MA3-027 was used in immunocytochemistry to study how the stop tranfer sequence regulates ER residency of UGT1A
|Barré L,Magdalou J,Netter P,Fournel-Gigleux S,Ouzzine M||The FEBS journal (272:1063)||2005|
Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER.
MA3-027 was used in immunocytochemistry to investigate the endoplasmic reticulum-associated degradation of channel proteins .
|Khanna R,Lee EJ,Papazian DM||Journal of cell science (117:2897)||2004|
An LQT mutant minK alters KvLQT1 trafficking.
MA3-027 was used in immunocytochemistry to study the effect of the minK LQT mutation
|Krumerman A,Gao X,Bian JS,Melman YF,Kagan A,McDonald TV||American journal of physiology. Cell physiology (286:C1453)||2004|
Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator.
MA3-027 was used in immunocytochemistry to investigate the role of Csp in regulating CFTR trafficking at the plasma membrane.
|Zhang H,Peters KW,Sun F,Marino CR,Lang J,Burgoyne RD,Frizzell RA||The Journal of biological chemistry (277:28948)||2002|
|Non-human primate||Not Cited||
Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm.
MA3-027 was used in immunocytochemistry to study the disulfide bond formation during simian virus 40 Vp1 oligomerization
|Li PP,Nakanishi A,Clark SW,Kasamatsu H||Proceedings of the National Academy of Sciences of the United States of America (99:1353)||2002|
CTP:phosphocholine cytidylyltransferase alpha is a cytosolic protein in pulmonary epithelial cells and tissues.
MA3-027 was used in immunocytochemistry to examine the expression of CTP:phosphocholine cytidylyltransferase alpha in pulmonary epithelial cells and tissues.
|Ridsdale R,Tseu I,Wang J,Post M||The Journal of biological chemistry (276:49148)||2001|
Glycosylation increases potassium channel stability and surface expression in mammalian cells.
MA3-027 was used in immunocytochemistry to demonstrate the effect of glycosylation on Shaker protein stability and surface expression in mammalian cells.
|Khanna R,Myers MP,Lainé M,Papazian DM||The Journal of biological chemistry (276:34028)||2001|
The Hdj-2/Hsc70 chaperone pair facilitates early steps in CFTR biogenesis.
MA3-027 was used in immunocytochemistry to investigate the possible roles of Hdj-2 in directing Hsc70 to facilitate the assembly of cytosolic regions on CFTR
|Meacham GC,Lu Z,King S,Sorscher E,Tousson A,Cyr DM||The EMBO journal (18:1492)||1999|
Limitations of In Vivo Reprogramming to Dopaminergic Neurons via a Tricistronic Strategy.
MA3-027 was used in immunohistochemistry - frozen section to identify somatic cell types in vivo that can be reprogrammed
|Theodorou M,Rauser B,Zhang J,Prakash N,Wurst W,Schick JA||Human gene therapy methods (26:107)||2015|
Connexins modulate autophagosome biogenesis.
MA3-027 was used in western blot to study the regulation of autophagosome biogenesis by connexins
|Bejarano E,Yuste A,Patel B,Stout RF,Spray DC,Cuervo AM||Nature cell biology (16:401)||2014|
Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
MA3-027 was used in western blot to study the role of NPGPx as an oxidative stress sensor and its role in GPR78 chaperone activity
|Wei PC,Hsieh YH,Su MI,Jiang X,Hsu PH,Lo WT,Weng JY,Jeng YM,Wang JM,Chen PL,Chang YC,Lee KF,Tsai MD,Shew JY,Lee WH||Molecular cell (48:747)||2012|
Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
MA3-027 was used in western blot to identify NPGPx as a novel responder to non-targeting siRNA-induced stress
|Wei PC,Lo WT,Su MI,Shew JY,Lee WH||Nucleic acids research (40:323)||2012|
Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin.
MA3-027 was used in western blot to investigate the role of importin beta in intracellular protein degradation process
|Zhong Y,Wang Y,Yang H,Ballar P,Lee JG,Ye Y,Monteiro MJ,Fang S||The Journal of biological chemistry (286:33921)||2011|
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
MA3-027 was used in western blot to investigate the role of presenilin 1 in Lysosomal proteolysis and autophagy
|Lee JH,Yu WH,Kumar A,Lee S,Mohan PS,Peterhoff CM,Wolfe DM,Martinez-Vicente M,Massey AC,Sovak G,Uchiyama Y,Westaway D,Cuervo AM,Nixon RA||Cell (141:1146)||2010|
|Non-human primate||Not Cited||
A novel transmembrane domain mediating retention of a highly motile herpesvirus glycoprotein in the endoplasmic reticulum.
MA3-027 was used in western blot to identify the mechanism for the retention of a herpesvirus glycoprotein in the endoplasmic reticulum
|Däubner T,Fink A,Seitz A,Tenzer S,Müller J,Strand D,Seckert CK,Janssen C,Renzaho A,Grzimek NK,Simon CO,Ebert S,Reddehase MJ,Oehrlein-Karpi SA,Lemmermann NA||The Journal of general virology (91:1524)||2010|
West Nile virus infection activates the unfolded protein response, leading to CHOP induction and apoptosis.
MA3-027 was used in western blot to study the effect of West Nile virus infection on CHOP induction and apoptosis
|Medigeshi GR,Lancaster AM,Hirsch AJ,Briese T,Lipkin WI,Defilippis V,Früh K,Mason PW,Nikolich-Zugich J,Nelson JA||Journal of virology (81:10849)||2007|
Suberoylanilide hydroxamic acid (vorinostat) represses androgen receptor expression and acts synergistically with an androgen receptor antagonist to inhibit prostate cancer cell proliferation.
MA3-027 was used in western blot to study the effects of suberoylanilide hydroxamic acid (vorinostat) on androgen receptor expression and prostate cancer cell proliferation
|Marrocco DL,Tilley WD,Bianco-Miotto T,Evdokiou A,Scher HI,Rifkind RA,Marks PA,Richon VM,Butler LM||Molecular cancer therapeutics (6:51)||2007|
Isolation of lipid droplets from cells by density gradient centrifugation.
MA3-027 was used in western blot to evaluate a density gradient centrifugation protocol for the isolation of cellular lipid droplets
|Brasaemle DL,Wolins NE||Current protocols in cell biology (Chapter 3:null)||2006|
Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex.
MA3-027 was used in western blot to study the interaction of Sec13 and Nup96 during interphase.
|Enninga J,Levay A,Fontoura BM||Molecular and cellular biology (23:7271)||2003|
c-erbB-3: a nuclear protein in mammary epithelial cells.
MA3-027 was used in western blot to demonstrate the nuclear expression and localization of c-erbB-3 in MCF-7 cells
|Offterdinger M,Schöfer C,Weipoltshammer K,Grunt TW||The Journal of cell biology (157:929)||2002|
Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells.
MA3-027 was used in immunohistochemistry to investigate the functions of LGN and AGS3 in photoreceptor cells
|Kerov VS,Natochin M,Artemyev NO||Molecular and cellular neurosciences (28:485)||2005|
Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.
MA3-027 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.
|Calvert ME,Digilio LC,Herr JC,Coonrod SA||Reproductive biology and endocrinology : RB and E (1:null)||2003|
ER-60, a chaperone with thiol-dependent reductase activity involved in MHC class I assembly.
MA3-027 was used in immunoprecipitation to discover novel factors related to the assembly of MHC class I molecules
|Lindquist JA,Jensen ON,Mann M,Hämmerling GJ||The EMBO journal (17:2186)||1998|
Retention of unassembled components of integral membrane proteins by calnexin.
MA3-027 was used in immunoprecipitation to investigate calnexin's interaction with incompletely assembled TCR components
|Rajagopalan S,Xu Y,Brenner MB||Science (New York, N.Y.) (263:387)||1994|