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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Calpain protein purified from placenta.|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||3mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1.0 µg/ml|
|Immunoprecipitation (IP)||1-2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and m-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, m-calpain is active at
micromolar (mM) concentrations of Ca++. In addition to the ubiquitously expressed m- and m- calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
calcium-activated neutral proteinase 1; calcium-activated neutral proteinase 2; calpain 1, (mu/I) large subunit; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain M-type; calpain mu-type; calpain, large polypeptide L1; calpain, large polypeptide L2; calpain-1 large subunit; Calpain-2 catalytic subunit (EC:126.96.36.199); calpain-2 large subunit; CANP; CANP 1; CANP 2; CANP1; CANP2; CANPL1; cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; M-calpain; micromolar-calpain; millimolar-calpain; muCANP; muCL
CANP; CANP1; CANP2; CANPL1; CANPL2; CANPml; CAPN1; CAPN2; mCANP; muCANP; muCL; PIG30; SPG76