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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human calpain protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both calpain I and II and is associated with myotonic dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
30K; calcium-activated neutral proteinase small subunit; calcium-dependent protease; calcium-dependent protease small subunit 1; calcium-dependent protease, small subunit; calpain; calpain 4; calpain 4, small subunit (30K); calpain regulatory subunit; calpain small subunit 1; calpain, small polypeptide; calpain, small subunit 1; CALPAIN4; CANP; CANP small subunit; CANPS; CAPN4; CDPS; CSS1; small pol; small subunit; small subunit 30K
CALPAIN4; CANP; CANPS; Capa-4; Capa4; CAPN4; CAPNS; CAPNS1; CDPS; CSS1; D7Ertd146e