Invitrogen

Calpain S1 Monoclonal Antibody (156)

Product Image Gallery

Calpain S1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Calpain in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Calpain monoclonal antibody (Product # MA3-943) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10 - 1:100

Product Specifications

Species Reactivity

Bovine, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

156

Immunogen

Purified bovine skeletal muscle 28 kDa calpain subunit.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites diluted in PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2069853

Product Specific Information

MA3-943 detects calpain-28 kDa subunit from bovine platelet, skeletal and heart muscle and human tissues. This antibody does not cross-react with the human 80 kDa m-calpain subunit.

MA3-943 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 28 kDa protein representing the 28 kDa subunit of calpain from bovine heart extract.

The MA3-943 antigen is purified bovine skeletal muscle 28 kDa calpain subunit.

Target Information

Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both calpain I and II and is associated with myotonic dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 30K; Calcium-activated neutral proteinase small subunit; Calcium-dependent protease small subunit; Calcium-dependent protease small subunit 1; calcium-dependent protease, small subunit; calpain 4, small subunit (30K); calpain II regulatory subunit; calpain II regulatory subunit (EC 3.4.22.17); Calpain regulatory subunit; Calpain small subunit 1; calpain, small polypeptide; calpain, small subunit 1; CANP small subunit; CDPS; CSS1; small pol

Gene Aliases: CALPAIN4; CANP; CANPS; CAPN4; CAPNS; CAPNS1; CDPS; CSS1

UniProt ID: (Human) P04632

Entrez Gene ID: (Human) 826, (Bovine) 281664

Molecular Function: annexin calcium-binding protein calmodulin cysteine protease hydrolase intracellular calcium-sensing protein protease