MA3-945 detects calpastatin from bovine, human, canine, pig, rabbit, and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin.
MA3-945 has been successfully used in Western blot, immunohistochemistry, and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells with MA3-945 results in diffuse cytoplasmic staining and weak nuclear staining.
The MA3-945 antigen is bovine skeletal muscle calpastatin. This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human calpastatin.
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calpain inhibitor; Calpastatin; calpastatin isoform III; calpastatin precursor (EC 184.108.40.206); calpastatin type 1; calpastatin type 2; calpastatin type I; calpastatin type II; calpastatin type III; calpastatin type IV; Erythrocyte calpastatin; heart calpastatin; Sperm BS-17 component
Gene Aliases: BS-17; CAST; PLACK