Immunofluorescence analysis of Calponin-1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Calponin-1 (CALP) Mouse Monoclonal Antibody (Product # MA5-11620) at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the cytoskeleton. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Crude human uterus extract|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:800-1600|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11620 targets Calponin-1 in IHC (P) applications and shows reactivity with Human and Rat samples.
The MA5-11620 immunogen is crude human uterus extract.
Calponin, a calmodulin, binds tropomyosin and F-actin and is thought to be involved in the regulation of smooth muscle contraction. The expression of calponin is restricted to smooth muscle cells and is a marker of the differentiated contractile phenotype of developing smooth muscle. Two isoforms of calponin exist whose molecular weights are 34kDa and 29kDa. Expression of the 29kDa form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Metastatic inflammatory myofibroblastic tumor identified by EUS-FNA in mediastinal lymph nodes with ancillary FISH studies for ALK rearrangement.
MA5-11620 was used in immunohistochemistry to investigate ALK gene rearrangement in a clinical case of metastatic pulmonary inflammatory myofibroblastic tumor
|Borak S,Siegal GP,Reddy V,Jhala N,Jhala D||Diagnostic cytopathology (40 Suppl 2:E118)||2012|
Pericardial synovial sarcoma, a potential for misdiagnosis: clinicopathologic and molecular cytogenetic analysis of three cases with literature review.
MA5-11620 was used in immunohistochemistry to report on three cases of pericardial synovial sarcoma
|Cheng Y,Sheng W,Zhou X,Wang J||American journal of clinical pathology (137:142)||2012|
TGF-beta1 drives partial myofibroblastic differentiation in chondromyxoid fibroma of bone.
MA5-11620 was used in immunohistochemistry to study the role of TGF-beta in myofibroblastic differentiation in chondromyxoid fibroma
|Romeo S,Eyden B,Prins FA,Briaire-de Bruijn IH,Taminiau AH,Hogendoorn PC||The Journal of pathology (208:26)||2006|
Identification of steroid sulfate transport processes in the human mammary gland.
MA5-11620 was used in immunohistochemistry to study steroid sulfate transport processes in the human mammary gland.
|Pizzagalli F,Varga Z,Huber RD,Folkers G,Meier PJ,St-Pierre MV||The Journal of clinical endocrinology and metabolism (88:3902)||2003|
Modulation of gene expression in precancerous rat esophagus by dietary zinc deficit and replenishment.
MA5-11620 was used in western blot to study the effect of dietary zinc on gene expression in precancerous rat esophagus
|Liu CG,Zhang L,Jiang Y,Chatterjee D,Croce CM,Huebner K,Fong LY||Cancer research (65:7790)||2005|