This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species.
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase prior to IHC staining. A suggested positive control is human spleen. For flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.
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Protein Aliases: BEE22; calgranulin B; Calgranulin-B; calprotectin; Calprotectin L1H subunit; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; p14; p23; Protein S100-A9; RNA-binding region containing protein 2-like; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)
Gene Aliases: 60B8AG; BOS_2815; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14; NIF; P14; S100A9