|Tested species reactivity||Hamster, Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Calreticulin-maltose binding fusion protein|
|Storage buffer||PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (IHC)||2 µg/ml|
|Immunoprecipitation (IP)||12.5 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91034 detects Calreticulin from human, monkey, and hamster samples.
MA1-91034 has been successfully used in FACS, IP, immunocytochemistry, immunohistochemistry, Western blot applications.
The MA1-91034 immunogen is a calreticulin-maltose binding fusion protein
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles
Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscles SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptor and may also act as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.